炎性痛诱导的大鼠背根节及脊髓后角NMDAR1表达变化的研究  

Inflammation-induced Changes of NMAD Receptor Eexpression in Rat's DRG and Spinal Cord

作　　者：陈世新[1] 廖敏[1] 

机构地区：[1]咸宁学院生物医学工程系,湖北咸宁437100

出　　处：《咸宁学院学报（医学版）》2004年第3期171-174,共4页Journal of Xianning Univarsity(medical Sciences)

摘　　要：目的 观察炎性痛条件下NMDA受体亚型R1(NR1)的表达变化。方法 采用福尔马林试验,在大鼠一侧足背皮下注射5％福尔马林50μl制备局部炎性痛病灶,利用痛行为测定结合组织化学方法检测大鼠背根节及脊髓后角NR1的表达变化。结果 炎症组大鼠背根节中、小型细胞及脊髓背角的NR1表达较生理盐水组显著增加,表明福尔马林刺激导致的炎症痛及痛过敏可能是通过上调NR1受体的表达而实现的。结论 NMDA受体的激活在痛觉过敏的产生和维持中起着至关重要的作用。Objective To observe the expression changes of NMDAR1 (NR1) under inflammatory pain. Methods Histochemistry technique associated with pain-behavior-rating were applied to observe the expressionchanges of the RA1 in the dorsal mot ganglion(DRG) and spinal cord following the injection of 5% formalin 50μl. Results It was found that the NR1 expression in DRG and dorsal horn of L4-5 segment of the formalin group was significantly stronger than that in the normal saline group. Conclusion The results Suggested that the up-regulation and activation of NR1 in the DRG and spinal dorsal horn maybe significantly contributeto the hyperalgesia that accompanies inflammation.

关 键 词：炎性痛 大鼠 背根节 脊髓后角 基因表达 福尔马林 N-甲基-D-天门冬氨酸 

分 类 号：R441.1[医药卫生—诊断学] R339.1[医药卫生—临床医学]