腺病毒介导的BMP-2基因转染骨髓基质干细胞及种植异种骨支架的体外观察  

Construction of tissue engineering bone with Ad-BMP-2 transfected bone marrow stromal cells and BCB scaffold in vitro

作　　者：景元海[1] 臧虎[2] 符强[1] 许文彬[1] 朱庆三[2] 

机构地区：[1]大庆龙南医院骨科,黑龙江大庆市163453 [2]吉林大学中日联谊医院骨科

出　　处：《中国实验诊断学》2004年第4期391-393,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(39800151);吉林省科技厅基金资助项目(20010110)

摘　　要：目的　用人骨形态发生蛋白 2腺病毒表达载体 (Ad BMP 2 )转染的兔骨髓基质干细胞 (BMSC) ,种植BCB(去抗原牛松质骨 )支架体外构建组织工程骨。方法　蛋白印迹法检测转染后细胞BMP 2的表达 ,ALP活性检测分析基因转染对细胞分化的影响。然后将转染后细胞接种到BCB支架上 ,扫描电镜观察细胞贴附、生长状况。结果　转染后 ,BMSC表达BMP 2 ,ALP活性明显增高。扫描电镜见转染细胞分布均匀 ,伸展良好。结论　Ad BMP 2可高效转染BM SC ,且促进细胞分化。转染后细胞在BCB上生长良好 ,BMPObjective Rabit bone marrow stromal cells(BMSC) infected by a recombinant adenoviral vector carrying the human BMP 2 gene(Ad BMP 2) were seeded into BCB scaffolds to construct tissue engineering bone in vitro.Methods The expression of hBMP 2 in these cells after infection was determined by Western blot analysis. The changes of cellular differentiation were observed by ALP activity analysis. BMP 2 transduced cells were then seeded into BCB scaffolds. The attachment and growth of the cells on the scaffold were examined using SEM.Results The expression of hBMP 2 was confirmed and ALP activity obviously increased in the cells after infection. SEM examination revealed extensive cellular attachment and growth on the BCB block.Conclusion Ad BMP 2 could infect BMSC with high efficiency and promote its differentiation. The cells after infection grew well on a BCB scaffold. Tissue engineering bone used to regional gene therapy was constructed successfully.

关 键 词：腺病毒 骨形态发生蛋白 骨髓基质干细胞 组织工程骨 

分 类 号：R687[医药卫生—骨科学]