钙离子电极研究金属离子与植物钙调素的竞争结合作用  

Study on the Metal Ion Competition Binding Abilities to CaM by Ca^(2+)--Selective Electrode

作　　者：肖凤娟[1] 常虹[2] 

机构地区：[1]石家庄铁道学院材料科学与工程系,河北石家庄050043 [2]河北师范大学物理学学院,河北石家庄050016

出　　处：《河北化工》2004年第4期32-34,共3页Hebei Chemical Industry

基　　金：河北省自然科学基金项目(98207)

摘　　要：利用钙离子选择电极研究了La3+、Cu2+、Zn2+、Cd2+、Mg2+和Al3+等金属离子与拟南芥钙调素(CaM)的竞争结合作用。结果表明:La3+、Cu2+、Zn2+、Cd2+与钙调素的竞争结合能力较强,Al3+和Mg2+的竞争能力较弱。竞争实验的结果从分子水平上证明了金属离子可取代Ca2+4·CaM中的Ca2+与钙调素产生竞争性结合,揭示了这些金属离子生物效应可能的分子机制。The effects of metal ions La3+,Cu2+,Zn2+,Cd2+,Mg2+and Al3+ on the substrate-binding ability of cauliflower calmodulin have been studied by Ca2+ selective electrode .The competition binding abilities of La2+,Cu2+,Zn2+,Cd2+ to CaM are higher than that of Al3+ and Mg2+.The results comfirmed that metal ions can substrate Ca2+ in Ca42+·CaM system and bind to calmodulin competively,meanwile,the experiment has disclosed the molecular mechanism of the biological effects of La3+, Cu2+, Zn2+,Cd2+,Al3+and Mg2+ at molecular level.

关 键 词：钙离子选择电极 金属离子 CAM 竞争结合 

分 类 号：Q51[生物学—生物化学]