情绪应激对NOD小鼠血糖、胰岛炎及胰岛内细胞凋亡的影响  

The effect of emotional stress on the intra-islet apoptosis

作　　者：陶嵘[1] 姚树桥[1] 赵勇[1] 石川[1] 张式兴[1] 

机构地区：[1]中南大学湘雅二医院医学心理学研究中心,长沙410011

出　　处：《中国行为医学科学》2004年第4期372-374,共3页Chinese Journal of Behavioral Medical Science

基　　金：湖南省自然科学基金资助项目 (OOJJY2 0 70 )

摘　　要：目的探讨情绪应激在NOD鼠 1型糖尿病发生中的作用及可能机制。方法采用饮水焦虑冲突应激及限制应激合用 ,对 2月龄NOD小鼠进行急性应激作用 ,研究应激 1d ,2d及 3d后 ,小鼠的情绪性、血糖、胰岛炎及胰岛内细胞凋亡水平与对照组之间的差异和时间趋势。结果应激 1d后小鼠情绪唤醒和血糖水平即升高 ,从开始的 (2 .0 0± 1.41)mmol/L和 (3 .2 7± 0 .70 )mmol/L上升至 (4 .67± 1.3 7)mmol/L和 (8.68± 4.63 )mmol/L ,并随着应激时间的延长有逐步下降的趋势。而胰岛炎和胰岛细胞凋亡水平则随着应激时间的延长逐步升高 ,平均每胰岛的胰岛炎积分从 0 .16逐渐上升到应激 3d后的 0 .75 ,平均每胰岛凋亡细胞个数则从对照组的 0 .82± 0 .93上升为 3 .93± 1.0 5 (应激 1d)、5 .72± 3 .90 (应激 2d)、5 .77± 2 .75 (应激 3d)。结论 1、2、3d的饮水焦虑冲突应激及限制应激可增加NOD鼠胰岛炎和胰岛细胞凋亡水平 ,从而可能促进Objective To explore the effect of emotional stress on the onset of type 1 diabetes and it's possible mechanism. Methods Using the drinking-conflict stress and restraint stress, we investigated diabetses-prone NOD mouse's emotionality, blood glucose, insulitis and intra-islet apoptosis. Time couse of these indexes are also studied. Results The emotionality level and glucose level were increased after stress, from (2.00 ±1.41 )mmol/Land(3.27±0.70)mmol/L to (4.67±1.37)mmol/L and (8.68±4.63) mmol/L, but the increased level showed a decreasing trend with time. Meanwhile, insulitis and apoptosis level increased with time, the average insulitis score increased from 0.16 to 0.75 gradually.The average intra-islet apoptosis increased from (0.82±0.93) to (3.93±1.05)(5.72±3.90)(5.77±2.75) Conclusionsone ,two or three days'drinking-conflict stress and restraint stress can increase insulitis and intra-islet apoptosis level of NOD mice, which may contribute to the onset of type 1 diabetes.

关 键 词：情绪应激 1型糖尿病 胰岛炎 细胞凋亡 血糖 小鼠 

分 类 号：R395.1[哲学宗教—心理学]