饮用水中几种细菌计数方法的比较  

Methods of Enumeration of Bacteria in Drinking Water

作　　者：鲁巍[1] 王云[1] 张晓健[1] 

机构地区：[1]清华大学环境科学与工程系,北京100084

出　　处：《环境科学》2004年第4期167-169,共3页Environmental Science

基　　金：国家自然科学基金资助项目 ( 5 0 2 3 80 2 0 ) ;国家高技术研究发展计划 ( 863计划 )资助项目 ( 2 0 0 2AA60 114 0 )

摘　　要：比较采用不同培养基的平板计数 (PlateCounts ,PC)方法 ,以及不同荧光染色剂的显微镜直接计数方法与常规计数方法的差别 .研究认为 ,常规平板计数方法并不能准确反映饮用水中实际存活的细菌数量 ;吖啶橙直接计数 (AcridineOrangeDirectCounts,AODC)的结果最高 ,较常规平板计数方法高出 3～ 4个量级 ;活细菌直接计数 (DirectViableCounts,DVC)中 ,DVC N .A .、DVC CTC和DVC BacLight等计数方法的结果较常规平板计数结果高出 2～ 3个量级 .以地表水为水源的水厂出水中活细菌数占总细菌数的比例在 1 0Methods of the enumeration of total bacteria and Coliform in drinking water were researched in this paper. The differences between heterotrophic plate count and direct viable count method were compared. It is concluded that the total number of bacteria in R2A medium is one order of quantity higher than the traditional plate count agar, and the results of acridine orange direct counts(AODC) is the highest. Some different staining methods in direct viable count were also compared in this paper, such as nalidixic acid, CTC staining and BacLight staining. The proportion of the live bacteria to the dead is about 10%.

关 键 词：饮用水 直接计数 平板计数 活细菌直接计数 细菌 

分 类 号：X832[环境科学与工程—环境工程] TU991.25[建筑科学—市政工程]