骨形态发生蛋白在微小颗粒骨诱导成骨过程中的表达  

Expression and significance of BMP-2 in autogenesis of minimal morselized bone during ectopic osteogenesis

作　　者：张滨[1] 闫景龙[1] 夏景君[1] 王洪伟[1] 汪宇[1] 

机构地区：[1]哈尔滨医科大学附属第二医院脊柱关节外科,150086

出　　处：《中国矫形外科杂志》2004年第16期1247-1250,共4页Orthopedic Journal of China

摘　　要：目的 :探讨自体微小颗粒骨在诱导成骨过程中骨形态发生蛋白 (BMP)的表达及成骨机制。方法 :日本大耳白兔 48只 ,随机分为微小颗粒骨 (A组 )、块状骨 (B组 )两组 ,每组 2 4只 ,取左侧髂骨 ,修剪成 0 .3cm× 0 .5cm×l.0cm骨块 ,A组将骨块制成微小颗粒骨 ,B组骨块不处理 ,均植入右侧臀大肌肌袋 ,于术后 1、3、5、7、1l、14、2 1、2 8d取材 (包括移植物及周围软组织 ) ,做组织学、免疫组化、原位杂交染色 ,显微镜下观察结果并做计算机图像分析。结果 :( 1)在异位成骨过程中 ,微小颗粒骨吸收迅速、替代完全 ,BMP释放量大 ;( 2 )骨诱导伴随着成骨的全过程 ,BMP在其中发挥重要作用 ;( 3 )微小颗粒骨可能起到骨化中心的作用。结论 :自体微小颗粒骨异位成骨强于块状骨 。Objective:To study the expression of BMP during osteogenesis of autogenesis of minimal morselized bone (AMMB) and its mechanism. Method:Forty eight rabbits were divided into 2 groups equally and randomly :bulk bone was harvested from left ilium of rabbits. In group A(24 rabbits): bulk bone was turned into morselized bone (0.3 ×0.5×1.0)cm 3;in group B(24 rabbits): bulk bone was planted in right musculus glutaeus maximus .Sample was havested at the 1 st , 3 rd , 5 th , 7 th , 9 th ,11 th , 14 th , 21 st , 28 th day after operation. Then it was tested by histological method, immunohistological chemistry and ISH. All the result was analyzed by computer. Result: (1) During ectopic osteogenesis of AMMB was absorbed quickly and replaced totally, compared with group B,the concentration of BMP in group A was higher, (2) BMP existed and played the key role during the whole course of osteogenesis. (3) AMMB may be the origin of osteogenesis. Conclusion:The ectopic osteogenesis of AMMB is better than that of bulk bone, and BMP is main factor during osteogenesis.

关 键 词：自体微小颗粒骨 骨形态发生蛋白 诱导成骨 表达 

分 类 号：R687[医药卫生—骨科学]