中枢神经系统表达TPO受体的初步研究  

Identification of TPO Receptors on Central Nervous System——A Preliminary Report

作　　者：杨默[1] 夏文杰[1] 李桂霞[1] 庞雅轩[1] 戚其威[1] 李志光[1] 吴香玲[2] 吴浩强[2] 冯国培[3] 霍泰辉[1] 

机构地区：[1]香港中文大学儿科系 [2]香港中文大学病理系 [3]香港中文大学中医中药研究所

出　　处：《中国实验血液学杂志》2004年第4期494-497,共4页Journal of Experimental Hematology

基　　金：the RGC Direct Grant,The Government of Hong Kong.

摘　　要：为了研究中枢神经系统是否表达促血小板生成素 (TPO)的受体c mpl和TPO对神经细胞生长和保护作用 ,用免疫组织化学和RT PCR方法分析c mpl表达 ,用MTT ,annexinⅤ方法和流式细胞术检测、观察TPO对神经细胞生长和抗凋亡作用。结果表明 ,人中枢神经系统和小鼠神经细胞均表达TPO受体c mpl,人大脑半球和小脑及小鼠神经干细胞株C17.2在mRNA水平表达c mpl,进一步在蛋白水平证实TPO受体c mpl在人大脑半球神经细胞表达 ,TPO对C17.2细胞有生长促进作用和抗凋亡作用。结论 :本研究首次证实中枢神经系统表达TPO受体c mpl和TPO有促进体外神经细胞生长和抗凋亡的作用。To identify the expression of thrombopoietin (TPO) receptors (c-mpl) on central nervous system (CNS) and to evaluate the role of TPO on neural cell proliferation and protection,immunohistochemical staining,RT-PCR,MTT,and annexin-V methods were used in this study. The results showed the expression of TPO receptor on human CNS and murine neural cells. C-mpl mRNA was identified in human cerebral hemispheres and cerebellum,and mouse neural cell line C17.2 by RT-PCR. C-mpl was also confirmed in human cerebral hemispheres by immunohistostaining with con-focal microscopy. Furthermore,TPO had a stimulating effect on the growth of in vitro neural cell C17.2 by MTT assay. The anti-apoptotic effect of TPO on C17.2 cells was also demonstrated by staining with annexin-V and PI. In conclusion,the first evidence showed the expression of TPO receptor c-mpl in central nervous system. Moreover,the effect of TPO on neural cell proliferation and anti-apoptosis was also demonstrated on in vitro neural cells.

关 键 词：血小板生成素 c—mpl 中枢神经系统 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R973[医药卫生—基础医学]