卵子第一极体、卵周隙及胞浆形态与ICSI后受精率和胚胎质量的影响  

Oocyte morphology based on first polar body, perivitelline space and cytoplasm correlation with fertilization rate and embryo quality after intracytoplasmic sperm injection.

作　　者：邹淑花[1] 李凤兰[1] 吴瑞英[1] 王晓燕[1] 毕媛[1] 王海涛[1] 张全[1] 

机构地区：[1]青岛市妇女儿童医疗保健中心生殖中心,山东266012

出　　处：《中国优生与遗传杂志》2004年第4期113-115,104,共4页Chinese Journal of Birth Health & Heredity

摘　　要：目的　从MII期卵子的第一极体、卵周隙和胞浆形态三方面综合探讨卵子质量对卵母细胞浆内单精子注射(ICSI)后的受精率、卵裂率、优胚获得率的影响。方法　对ICSI中去除颗粒细胞后的MII期卵子在光镜下从第一极体、卵周隙和胞浆形态三方面综合评价卵子质量并按形态表现分为五级。且分别比较各级卵子的受精率、卵裂率及优胚率。结果　Ⅰ级卵子与其他各级含异常形态的卵子比较 ,受精率、卵裂率和优质胚胎率 ,均有显著差异 (P <0 .0 1)。Ⅰ级卵子有最高的受精率 (91.14 % )、卵裂率 (96 .35 % )和优质胚胎率 (83.91% )。形态异常的卵子的受精率、卵裂率和优质胚胎率均有明显的下降趋势。在取卵数≤ 15个及hCG注射日每个卵泡的血清E2浓度在 732～ 10 98pmol/L组中 ,可获得高比例的Ⅰ级卵子。结论　根据MII期卵子的第一极体、卵周隙及胞浆形态三方面综合因素进行的卵子分级是可预测ICSI后受精率、卵裂率及优胚率。Objective: To investigate the correlation of oocyte quality based on first polar body, perivitelline space and cytoplasm and fertilization rate, cleavage rate, grade 1-2 embryo rate after intracytoplasmic sperm injection. Methods: Oocyte at metaphase II were evaluated with oocyte morphology based on first polar body, perivitelline space and cytoplasm with light microscope after cumulus removed. Oocyte at metaphase II were graded into five groups. Graded oocytes were compared with each other from fertilization rate, cleavage rate, grade 1-2 embryo rate. Results: The oocytes at grade I had a significant higher fertilization rate, cleavage rate and grade 1-2 embryo rate than oocytes at others grades. (P<0.01). The oocytes at grade I had the highest fertilization rate(91.14%), cleavage rate(96.35%)and grade 1-2 embryo rate(83.91%). The fertilization rate, cleavage rate and grade 1-2 embryo rate of oocytes abnormality decreased significantly. The proportion of grade I oocytes, the fertilization rate, the cleavage rate and the grade 1-2 embryo rate were higher when the total number of oocytes retrieved was ≤15 and the oestradiol concentration was 732～1698pmol/l. Conclusions Human oocytes grading based on first polar body, perivitelline space and cytoplasmic morphology correlated with fertilization rate, cleavage rate and grade 1-2 embryo rate.

关 键 词：卵子 ICSI 胚胎质量 

分 类 号：R711.6[医药卫生—妇产科学]