孕早期干预CD80/CD86协同刺激信号对自然流产模型免疫活性细胞协同刺激分子表达的影响  

Effects of blocking in vivo CD80/CD86 costimulatory signal at the early gestation on the costimulatory molecules expression of immuno-competent cells in the abortion-prone model

作　　者：周跃华[1] 朱晓勇[1] 李大金[1] 朱影[1] 王明雁[1] 袁敏敏[1] 孟毅[1] 

机构地区：[1]复旦大学附属妇产科研究所生殖免疫研究室,上海200011

出　　处：《中国免疫学杂志》2004年第8期537-539,543,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金 ( 3 9770 773;3 0 2 0 0 2 99);复旦大学"985工程"项目( 985B3 6);计划生育药具国家重点实验室课题 (B2 0 2 1)资助

摘　　要：目的 :探讨孕早期干预协同刺激信号对自然流产模型孕鼠免疫活性细胞MHC Ⅱ类抗原和协同刺激分子的调控作用。方法 :建立自然流产模型组CBA×DBA 2和协同刺激信号干预组CBA×DBA 2 (CBA小鼠在孕 4天腹腔注射CD80及CD86mAb各 0 1mg)。分别于孕第 14天计数两组的胚胎吸收率 ,于孕第 9天采用流式细胞技术分析孕鼠脾细胞MHC Ⅱ类抗原与协同刺激分子CD80 (B7 1)、CD86 (B7 2 )、CD2 8和CD15 2 (CTLA 4 )的表达。结果 :孕早期干预协同刺激信号后 ,孕第 14天的胚胎吸收率显著下降 (P <0 0 5 ) ;孕第 9天免疫活性细胞CD80、CD86和CD2 8的表达显著下降 (P <0 0 1) ;CTLA 4的表达则显著升高 (P <0 0 5 ) ;而MHC Ⅱ类抗原的表达无显著改变 (P >0 0 5 )。结论 :孕早期体内干预协同刺激信号使免疫活性细胞协同刺激分子CD80、CD86、CD2 8表达下调和CTLA 4表达上调 ,及母胎界面Th2型免疫偏离 ,从而诱导系统性母Objective:To explore the effect of blocking the costimulatory signal at the early gestation on expression of costimulatory molecules and MHC-Ⅱ class antigen of immuno-competent cells in the abortion-prone model.Methods:After the abortion-prone CBA×DBA/2 matings were established,the pregnant CBA females were intraperitoeally injected with purified rat anti-mouse CD86/CD80mAb 0.1 mg at day 4 of gestation.Expressions of MHC-Ⅱ antigen and costimulatory molecules on the immuno-competent cells of spleen were analyzed by flow cytometry(FCM)at day 9 of gestation respectively.The embryo resorbing rate was counted at day 14 of gestation.Results:After in vivo blocking the costimulatory signal in the abortion-prone model,the embryo resorbing rate was reduced significantly(P<0.05),the expression of costimulatory molecules CD80,CD86 and CD28 on the splenic immuno-competent cells decreased significantly(P<0.01),while that of CTLA-4 increased significantly(P<0.05),that of MHC-Ⅱ antigen was invariable.Conclusion:The systemic materno-fetal immune tolerance induced by blocking the costimulatory signal may be attributed to the down-regulated expression of costimulatory molecules(CD80、CD86 and CD28)and up-regulated expression of CTLA-4 on the immuno-competent cells except for Th2 bias at the materno-fetal interface.

关 键 词：协同刺激分子 自然流产模型 胚胎吸收率 

分 类 号：R392.12[医药卫生—免疫学]