激活的人外周血及脐血树突状细胞对肿瘤细胞直接杀伤作用的比较  

The comparative study on the direct tumoricidal activity between activated human peripheral blood and cord blood dendritic cells

作　　者：石军[1] 池田和真[2] 藤井伸治[2] 金田衣代[2] 李晓[1] 浦权[1] 

机构地区：[1]上海交通大学附属第六人民医院血液科,上海200233 [2]日本冈山大学附属医院第二内科

出　　处：《中国免疫学杂志》2004年第8期544-547,共4页Chinese Journal of Immunology

摘　　要：目的 :探索人外周血或脐带血树突状细胞在干扰素 γ(IFN γ)或细菌脂多糖 (LPS)激活前后对肿瘤细胞杀伤活性的影响。方法 :分离健康供者外周血或脐血单核细胞 ,用重组粒单细胞集落刺激因子 (GM CSF)、白介素 4 (IL 4 )或联合肿瘤坏死因子 (TNF α)将其分别诱导为外周血树突状细胞 (PbDC)及脐血树突状细胞 (CbDC) ,二者分别于诱导第 7天或第 11天在合成培养基中加入LPS或IFN γ继续培养 12小时 ,将其激活。用流式细胞仪检测DC表面共刺激分子的改变 ;同时 ,以Jurkat、HL6 0及Daudi为靶细胞 ,以不同效靶比与DC共同培养 18小时 ,采用51 Cr释放实验检测DC激活前后抗肿瘤活性的差异。结果 :①LPS及IFN γ可上调外周血及脐带血DC表面CD86、CD83的表达 ,以LPS刺激组更明显 ,但对CD1a的表达影响较小。②以PbDC为效应细胞 ,LPS或IFN γ可分别增强DC对Daudi或HL6 0的杀伤活性 ,在效靶比为 2 0 :1时与未加刺激因子对照组(Medium DC)相比差异有显著意义 (P <0 0 1)。然而 ,LPS DC对HL6 0、IFN DC对Daudi则无明显杀伤活性 ;但二者对Jurkat却均有杀伤作用。③以CbDC为效应细胞 ,LPS及IFN γ对其杀伤活性的调节作用与对PbDC的相似。但在未加刺激因子前 ,Cb DC可有效杀伤Jurkat细胞 ,而MediumObjective:The tumoricidal activity between peripheral blood and cord blood dendritic cell activated by IFN-γ or LPS was compared.Methods:The mononuclear cells collected from normal peripheral blood or cord blood were cultured with IL-4,GM-CSF or together with TNF-α to induce peripheral blood DC(PbDC) for 7 days,cord blood DC(CbDC) for 11 days,then cultured for another 12 hours in the absence or presence of IFN-γ or LPS.The change of costimulatory molecules such as CD86,CD83 and CD1a were analyzed by flowcytometry.The cytotoxicity of stimulated DC by IFN-γ or LPS and unstimulated DC was measured with 51Cr release assay, in which Jurkat、HL60 and Daudi used as target cells.Results:①CD83 and CD86 expression in PbDC and CbDC were up-regulation by LPS or IFN-γ,but CD1a was not.②LPS and IFN-γ enhanced cytotoxicity of PbDC against HL60 and Daudi respectively.When effector to target radio was 20:1,the cytotoxicity of LPS-DC and IFN-DC were superior to that of unsitimulated DC(medium-DC)(P<0.01).Contrary,IFN-DC and LPS-DC had little or no tumoricidal effect to Daudi and HL60.However,both LPS and IFN-γ enhanced the cytotoxic activity of DC against Jurkat.③LPS and IFN-γ similarly regulated CbDC, however,CbDC remained tumoricidal activity toward Jurkat before stimulated,whereas the same effect was not detected in medium-PbDC.Conclusion:PbDC and CbDC activated by LPS or IFN-γ had similar specific cytotoxicity against tumor cells.

关 键 词：外周血 脐血 树突状细胞 IFN-R LPS 肿瘤杀伤活性 

分 类 号：R392.12[医药卫生—免疫学]