马齿苋总黄酮对氧自由基引发人红细胞膜损伤的保护作用  

Protective effect of portulaca total flavone on oxidative damage of erythrocyte ghost by oxygen free radicals

作　　者：卢新华[1] 关章顺[1] 何军山[1] 谭斌[1] 李元建[2] 黄煌[1] 岳赛[1] 

机构地区：[1]湘南学院,湖南郴州423000 [2]中南大学湘雅医学院药学院,湖南长沙410008

出　　处：《中国药学杂志》2004年第8期587-589,共3页Chinese Pharmaceutical Journal

基　　金：湖南省卫生厅科研课题(编号:9880)

摘　　要：目的研究马齿苋总黄酮对超氧阴离子(O2)引发的人红细胞膜氧化损伤的保护作用。方法 采用邻苯三酚自氧化产生的O2引发人红细胞膜氧化损伤模型,研究马齿苋总黄酮对红细胞膜氧化损伤的影响。结果O2能引起红细胞膜脂质过氧化,丙二醛(MDA)含量、自氧化速率显著增加,膜脂流动性及膜封闭度下降。而红细胞膜经一定量马齿苋总黄酮(60,120,240,480μg·mL-1)预先处理后,膜MDA含量明显减少,自氧化速率明显降低,膜脂流动性和膜封闭度显著增高。结论马齿苋对氧自由基引发的人红细胞膜氧化损伤有一定的保护作用。OBJECTIVE: To study the protective effect of portulaca total flavone(PTF) on the oxidative damage of erythrocyte ghost. METHODS: Oxidative damage of the erythrocyte ghost was induced by superoxide anion(O2-.) produced via auto-oxidation of pyrogallic acid. The effect of PTF on oxidated erythrocyte ghost was observed by the measurement of the content of malonidial aldehyde (MDA), membrane fluidity, blocking degree and speed of auto-oxidation from pyrogallic acid. RESULTS: O2-. increased the content of MDA and speed of auto-oxidation, decreased membrance fluidity, alleviated blocking degree, and caused lipid peroxidalion of erythrocyte ghost. PTF decreased the content of MDA and speed of auto-oxidation markedly, improved the membrance fluidity and blocking degree significantly, and inhibited the lipid peroxidation. CONCLUSION: PTF has protective effect on the oxidative damage induced by Q2-. of erythrocyte ghost.

关 键 词：马齿苋总黄酮 氧自由基 红细胞膜 保护作用 邻苯三酚 过氧化损伤 保护作用 

分 类 号：R285[医药卫生—中药学]