肠缺血-再灌注期细胞凋亡及维拉帕米的影响  

Observation on Cell Apoptosis after Intestinal Ischemia-reperfusion and the Effect of Verapamil on Apoptosis in Mice

作　　者：邓劼[1] 潘扬[1] 冯昌宗[1] 袁平[1] 马思星[1] 

机构地区：[1]贵阳医学院附院外科,贵州贵阳550004

出　　处：《贵阳医学院学报》2004年第4期318-321,共4页Journal of Guiyang Medical College

摘　　要：目的 :观察肠缺血 -再灌注期肠道细胞凋亡及黄嘌呤氧化酶 (XO)活性变化及钙通道阻滞剂维拉帕米对肠缺血再灌注细胞凋亡的影响 ,探讨钙超载、氧自由基与细胞凋亡三者之间的关系。方法 :通过肠系膜上动脉夹闭和开放复制肠缺血 -再灌注模型 ,分别于缺血 30min、6 0min后 3h或 72h再灌注或给维拉帕米后缺血 -再灌注 ,行HE及TVNEL染色 ,观察组织学变化和细胞凋亡 ;比色法做小肠组织XO活性测定。设假手术组 (不夹闭血管 )为对照。结果 :(1 )肠缺血 -再灌注后各时间点均可观察到细胞凋亡现象 ,尤以缺血 6 0min再灌注 3h组最为明显 ;(2 )小肠组织XO的活性在缺血 30min再灌注 3h组与假手术组比较升高 ,具有统计学意义 ,在该组中细胞凋亡与组织学改变与对照组比较没有统计学意义 ;(3)在缺血 30min再灌注 3h组 ,维拉帕米治疗组与实验组比较 ,XO活性下降 ,具有统计学意义 ;在缺血 6 0min再灌注 3h组 ,维拉帕米治疗组与实验组比较 ,细胞凋亡百分数与组织学评分均有下降 ,具有统计学意义 ;(4 )在各组缺血后再灌注 72h ,未发现有迟发性肠坏死发生。结论 :肠缺血 -再灌注后小肠组织可发生细胞凋亡 ,其在小肠缺血 -再灌注损伤的发生中发挥着重要的作用 ;钙通道阻滞剂维拉帕米对肠缺血 -再灌注引起的小肠组织损伤具有保护?Objective: 1) To investigate small bowel cell apoptosis and the change of xanthine oxidase (XO) activity during ischemia reperfusion; 2) To evaluate the effect of verapamil, a calcium channel blockers (CCBs), on small bowel cell apoptosis during ischemia reperfusion, and 3) To study the relationship among intracellular calcium overload, oxygen derived free radicals and apoptosis. Methods: Intestinal ischemia reperfusion was initiated by clamping the superior mesenteric artery (SMA) for 30 or 60 min followed by reperfusion. An approximately 10 cm length of terminal ileum was taken after reperfusion. Histological change was assessed using a grading scale from 0 to 3. The degree of apoptosis was assessed with TUNEL staining ( terminal deoxynucleotydil transferase mediated deoxy uridine triphosphate nick end labeling) and qualified by calculating apoptosis cells/100 cells. The activity of xanthine oxidase was assayed by spectrophotometry. Results: 1) Apoptotic intestinal cells were observed in all groups after ischemia reperfusion. At ischemia 60 minutes plus reperfusion 3 hours group, percentage of apoptotic cells was significantly higher than sham operated group; 2) The activity of small bowel xanthine oxidase increased after 30 minutes of ischemia and 3 hours of reperfusion compared with sham operated. The percentage of apoptotic cells and histological change in this group were not significantly different from sham operated group; 3) Animals treated with verapamil had significantly lower activity of xanthine oxidase after 30 minutes of ischemia and 3 hours of reperfusion and had significantly less cell apoptosis and histological scores after 60 minutes of ischemia and 3 hours reperfusion than mice of untreated groups; 4) After 30 or 60 minutes of ischemia and 72 hours of reperfusion, delayed intestinal necrosis could not be found. Conclusions: 1) Apoptosis occurs after intestinal ischemia reperfusion, which plays an important role in intestinal ischemia reperfusion injury; 2) During intestinal ischemi

关 键 词：再灌注损伤 小肠 细胞凋亡 维拉帕米 大鼠 

分 类 号：R654.3[医药卫生—外科学]