一种新的真菌用基础培养基——维乐菜汁培养基  

A New Culture Medium for Fungi——Weile Juice Agar

作　　者：刘杏忠[1] 黄亦存[1] 沈崇尧[1] 

机构地区：[1]北京农业大学植保系,北京100094

出　　处：《贵州农学院学报》1993年第1期32-36,共5页

基　　金：国家自然科学基金资助项目

摘　　要：经对5个亚门的17种真菌试验表明:所有真菌在V-6培养基上的生长、产孢与在马铃薯葡萄糖培养基(PDA)上效果基本相同。V-6培养基尤其适合卵菌的生长和产孢,而高等真茵在V-6培养基上的色素及产孢比PDA上稍少。经过疫霉15种33个分离物的试验表明,V-6培养基比PDA更适合疫霉菌的生长和产孢。由于维乐复合菜汁为自然产品加工成的商品,不受自然产品的季节性限制,制作简便,因此V-6培养基可以用作培养真菌的一般培养基,尤其可作腐、疫霉的产孢培养基。Weile Juice Medium (named V-6 agar) was prepared with 227g Weile juice(product of a mixture containing tomato, carrot, spinach, celery, lettuce and wax gourd juice by Beijing Agric. Univ.), 3g CaCO_3 (centrifuge-cleared at 4000rpm. for 20 min) and 2% agar 1 liter of water. The pH was adjusted to 6.0. In agar plate tests of 17 very diverse fungal species and 33 isolates of 15 Phytophthora species, the mycelial growth and sporuiation of species of oomyectes especially those of Phytophthora were better on V-6 agar than on potato dextrose agar(PDA), while the growth of other species was equally well on V-6 agar as well as on PDA. For sporuiation and pigmentation it was sligtly inferior on V-6 agar to PDA. The Weile broth (prepared similar to V-6 agar only without agar) has been satisfactorily used to culture fungi in our laboratory. V-6 agar is an useful, convenient and inexpensive culture medium for fungi.

关 键 词：维乐菜汁 培养基 

分 类 号：Q939.502[生物学—微生物学]