VEGF对人血单核细胞基质金属蛋白酶表达及活性的影响  

Effect of VEGF on expression and activity of MMPs of human monocytes

作　　者：王长谦[1] 汤大鸣[1] 王利民[1] 谢秀兰[1] 徐依敏[1] 丁泓毅[1] 宋伟[1] 王彬尧[1] 黄定九[1] 

机构地区：[1]上海第二医科大学附属仁济医院心内科,上海200001

出　　处：《心脏杂志》2004年第4期308-311,共4页Chinese Heart Journal

基　　金：上海市青年科技启明星计划 ( 98QB14 0 14 )资助

摘　　要：目的 :探讨血管内皮生长因子 (VEGF)对人单核细胞源巨噬细胞 (HMDM)基质金属蛋白酶 (MMPs)的表达及其活性的影响。方法 :分别应用 RT- PCR和 Western blot检测 MMPs(MMP- 2和 MMP- 9)基因和蛋白表达 ,Zym ography活性分析检测 MMPs活性。结果 :VEGF可明显增加人单核细胞源巨噬细胞 (HMDM) MMP- 2、MMP- 9m RNA及其蛋白表达和活性。结论 :VEGF可刺激 HMDM MMP- 2、MMP- 9m RNA及其蛋白的表达 ,增强其活性 ,因而有可能促进动脉粥样硬化斑块内基质降解 ,进而引起粥样硬化斑块破裂。AIM: To observe the effect of VEGF on expression and activitiy of Matrix Metalloproteinase(MMPs). METHODS: The gene and protein expression of MMPs(MMP-2 and MMP-9) were respectively detected by RT-PCR and Western blot. MMPs' activity was detected by Zymography. RESULTS: The expressions and activities of MMP-2 and MMP-9 mRNA of human monocytes were increased significantly by VEGF. CONCLUSION : As VEGF can stimulate the expressions and activities of MMP-2 and MMP-9 of human monocytes, it may increase the breakdown of matrix in lesions of atherosclerosis and enhance the lesions' vulnerability.

关 键 词：血管内皮生长因子 基质金属蛋白酶 动脉粥样硬化 

分 类 号：R541.4[医药卫生—心血管疾病]