免疫组化SP法操作程序的规范和改良  

Regulation and improvement of immunohistochemistry manipulation procedure

作　　者：陈罡[1] 侯巧燕[1] 陈峰[1] 张美艳[1] 

机构地区：[1]广西桂林临床病理诊断中心桂林医学院病理学教研室,桂林541001

出　　处：《医学文选》2004年第4期419-421,共3页Anthology of Medicine

摘　　要：目的　总结免疫组化 SP法的操作经验。方法　用改良后的 SP法对 1 2 0 0例病理组织切片进行免疫组化染色。结果　免疫组化标记定位准确 ,背景清晰者 1 1 2 0例 ,占总数 93 .3 %。结论　采用改良 SP法并注意操作要点 ,有助于提高免疫组化染色质量。Objective To summarize the experience in immunohistochemistry manipulation.Methods Staining 1 200 pathological sections with improved immunohistochemistry method.Results There was accurate localization with clear background in 1 120 sections,93.3% of the total.Conclusions It is helpful to improve the quality of immunohistochemistry using our method

关 键 词：免疫组化 抗原修复 

分 类 号：R446.8[医药卫生—诊断学]