银杏叶提取物对血管性痴呆大鼠海马胆碱能纤维的影响(英文)  

Effect of ginkgo biloba extract on cholinergic fibers in the hippocampus in rats of vascular dementia

作　　者：邓医宇[1] 孙诚[1] 曾红科[1] 胡道松[2] 茹立强[2] 张苏明[3] 

机构地区：[1]广东省人民医院急危重症医学部,广东省广州市510080 [2]华中科技大学同济医学院神经生物学教研室,湖北省武汉市430030 [3]华中科技大学同济医学院附属同济医院神经内科,湖北省武汉市430030

出　　处：《中国临床康复》2004年第25期5463-5465,F003,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:银杏叶提取物对血管性痴呆(vasculardementia,VD)大鼠的记忆功能及海马胆碱能纤维的影响,是目前国内外研究的热点,具有重大理论意义。目的:研究银杏叶提取物对VD的治疗作用及其机制。设计:随机对照实验研究。地点和对象:实验在华中科技大学同济医学院神经生物学教研室完成。实验动物采用纯种雄性Wistar大白鼠49只,体质量220～280g(华中科技大学实验动物中心供给)。干预:成年Wistar雄性大鼠49只随机分为假手术组(n=9)、VD模型组(n=40)。用避暗试验测定假手术组和VD模型组手术7d后的学习、记忆功能。将出现明显学习、记忆功能障碍的36只VD模型大鼠随机分为4组:模型对照组及银杏叶提取物低、中、高剂量组,每组9只动物,各组分别给予生理盐水1mL/100g、银杏叶提取物100,150,200mg/kg灌胃10d后,再用明、暗箱装置测定各组大鼠的学习、记忆功能。用胆碱酯酶染色观察了各组大鼠海马胆碱能纤维的密度。主要观察指标:①GBE对VD大鼠避暗试验的影响。②CA1区胆碱能纤维染色的结果。结果:与假手术组比较,模型组大鼠学习训练中错误次数明显增多,遭受电击的累积刺激时间(cumulatedstimulationtime,CST)明显延长;记忆测试中小鼠从放入明室到进入暗室遭受电击的步入潜伏期(stepthroughlatency,STL)明显缩短,错误次数?BACKGROUND:Ginkgo biloba extract(GBE) affects memory and cholinergic fibers in the hippocampus in VD rats,which is a hot topic of research at home and abroad at present and provides an important theoretical significance.OBJECTIVE:To study the therapeutic effect and its mechanism of GBE on vascular dementia.DESIGN:A randomized,controlled trial design.SETTING and MATERIALS:Research was completed at Neurobiology Department of Tongji Medical College,Huazhong University of Science and Technology.Male Wistar rats weighing 220-280 g obtained from experimental animal center of Huazhong University of Science and Technology were employed.INTERVENTION:Forty-nine male Wistar rats were divided into two groups:sham-operation group(n=9) and VD model groups(n=40).Seven days after operation,the rats were tested for learning and memory ability by passive avoidance conditioned response.Thirty-six VD model rats which were unable to acquire the passive avoidance response were divided into four group randomly:model control group,GBE low dosage group,GBE moderate dosage group,GBE high dosage group,9 rats for each group.The physiological saline 1 mL/100 g,GBE of 100,150 and 200 mg/kg were applied in each group respectively by gastric infusion for 10 days.After which,the learning and memory ability of rats in every group were determined by the illuminated and dark chambers;and the density of hippocampal cholinergic fibers tissues in rats in all groups were observed by acetylcholinesterase staining.MAIN OUTCOME MEASURES:①Effect of GBE on passive avoidance conditioned response in VD rats.②Results of cholinergic fiber staining in CA1 region. RESULTS:Compared with sham-operation group,in model group,during the learning test,the mistakes were remarkably increased and the cumulated stimulation time(CST) of electric shock were remarkably prolonged; During the memory test,the step through latency(STL) was remarkably shortened for the rats being shocked by electricity from an illuminated chamber to the dark chamber, the mistakes increased rem

关 键 词：银杏叶提取物 血管性痴呆 大鼠 海马胆碱能纤维 记忆功能 

分 类 号：R277.791.3[医药卫生—中医学]