低强度激光对大鼠实验性牙齿移动牙髓与牙周组织中P物质表达的影响(英文)  

Effect of low energy laser irradiation on the expression of substance P in dental pulp and periodontium during experimental orthodontic tooth movement in rats

作　　者：孙新华[1] 赵姝哲[1] 

机构地区：[1]吉林大学口腔医学院正畸科,吉林省长春市130041

出　　处：《中国临床康复》2004年第26期5738-5740,共3页Chinese Journal of Clinical Rehabilitation

基　　金：吉 林 省 自 然 科 学 基 金 资 助 项 目 (970322)~~

摘　　要：背景:目前国外的研究认为骨改建的过程可能部分地受神经肽调控。目的:观察低强度激光照射对大鼠实验性牙齿移动牙髓与牙周组织中P物质表达的影响。设计:随机对照的研究。地点和材料:于吉林大学第二临床学院动物室对45只Wistar大鼠进行动物实验。干预:利用免疫组化ABC染色,结合计算机图像分析,测定45只Wistar大鼠正畸牙齿移动在0,3,6,12,24和72h的实验侧和对照侧牙髓P物质免疫组化(SP-likeimmunoreactivity,SP-IR);以及在0(0h),1(24h),3(72h),7,14和21d时实验侧和对照侧牙周组织中SP-IR的灰度值。实验动物被分为9组,每组5只。于上颌第一磨牙和切牙之间拴结镍钛闭合螺旋弹簧,力值为40g。左侧为实验侧,右侧为对照侧。实验侧为低强度激光照射侧,采用He-Ne激光与CO激光同时照射的方法。2主要观察指标:①牙髓中SP-IR灰度值变化。②牙周膜压力区SP-IR灰度值变化。③牙周膜张力区SP-IR灰度值变化。④实验侧和对照侧的牙周膜压力区与张力区SP-IR灰度值变化的比较。结果:实验侧牙髓中SP-IR免疫组化灰度值低于对照侧,以第12,24h明显P<0.01);实验侧牙周膜压力区SP-IR免疫组化灰度值在7d时(明显高于对照侧(P<0.01);在14d时较高于对照侧(P<0.05)。实验侧牙周膜张力区SP-IR免疫组化灰度值在14d时较高于对照侧(P<0.05)。BACKGROUND:As believed by recent domestic and international researches,the bone reconstruction process might be partly modulated by neuropeptides. OBJECTIVE: To investigate the effect of low power laser irradiation on the expression of substance P (SP) in dental pulp and periodontium during experimental orthodontic tooth movement in rats. DESIGN:A randomized controlled trial SETTING and MATERIALS:Forty five Wistar rats were selected in the study in the animal laboratory of the Second Clinic Medicine College of Jilin University. INTERVENTIONS:The immunohistochemical reactivity of SP(SP IR) in dental pulp on both study side and control side of 45 Wistar rats with orthodontic tooth movement was assayed at 0,3,6,12,24,and 72 hours, as well as the gray scale of SP IR in periodontium on both study side and control side at 0 (0 hour),1(24 hours),3(72 hours), 7,14, and 21 days by immunohistochemical ABC staining combined with computerized image analysis system.Forty five Wistar rats were allocated into 9 groups according to experimental time of 0,3,6,12,24 hours,3 days,7 days, 14 days,and 21 days with 5 rats each.A NiTi coil spring with 40g connected the maxillary upper first molar to the upper incisor and fixed bilaterally by a ligature wire.The left side was set as study side and the right side was set as control side.Study side received low energy laser irradiation irradiated by He Ne laser and CO2 laser simultaneously. MAIN OUTCOME MEASURES:① SP IR gray scale alteration in dental pulp; ②SP IR gray scale alteration in periodontal pressure side;③ SP IR gray scale alteration in periodontal tension side; ④ the comparison of SP IR gray scale alterations at periodontal pressure and tension side between study side and control side. RESULTS:The dental pulp SP IR gray scale was lower in study side than that of control side, with most significant alteration at 12 hours, and 24 hours (P< 0.01). Periodontal pressure side SP IR gray scale in study side was significantly higher than that of control side at

关 键 词：激光 牙移动 P物质(SP) 牙髓 牙周组织 

分 类 号：R318[医药卫生—生物医学工程]