恙螨体内汉坦病毒核酸的基因芯片检测  

Detection for hantavirus in chigger mite with microarray techniques

作　　者：朱进[1] 陶开华[1] 操敏[1] 张云[1] 李越希[1] 唐家琪[1] 

机构地区：[1]南京军区军事医学研究所

出　　处：《中国公共卫生》2004年第3期268-270,共3页Chinese Journal of Public Health

基　　金：全军十五重点课题 ( 0 1L 0 0 6 )

摘　　要：目的　建立快速、灵敏、特异的汉坦病毒基因芯片诊断方法。方法　根据发表的汉坦病毒属 (HV ) 76-118株和R2 2株S基因核苷酸序列 ,设计引物和探针 ,制备寡核苷酸芯片。用Cy3标记核苷和引物 ,运用不对称PCR技术制备单链荧光标记核苷酸片段 ,并与芯片上的寡核苷酸探针杂交 ,荧光扫描仪检测并分析信号。结果　研究制备的基因芯片能够检测汉坦病毒HTN型和SEO型病毒核酸的特异性荧光信号。结论　HV的基因芯片检测具有特异、灵敏、快速的优点。基因芯片的制备和检测技术的建立 ,可为肾综合征出血热等传染病的诊断和预防提供理想的方法。ObjectiveTo establish a rapid,sensitive and specific dia gnostic assay for Hantavirus with microarray techniques. MethodsSpecific primers and probes were designed accordi ng to the conservative and specific DNA sequence of 76-118 strain and R22 strain .The probes were spotted on glass slides to form microarrays.The Cy3-labled sing le strand DNA fragment prepared by dissymmetical PCR were hybridized with the pr obers on the glass slides.The microarrays were scanned and analyzed with a scann er.ResultsDNA microarray could detect the different typed D NA of HTN and SEO with adequate specificity and sensitivity.ConclusionThe successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention,and widely applied in specific pathogen detection of infectious diseases such as hemorrha gic fever with renal syndrome.

关 键 词：肾综合征出血热 汉坦病毒 基因芯片 

分 类 号：Q811.4[生物学—生物工程]