硒拮抗氟对人肝细胞DNA损伤及凋亡的影响  

Effects of selenium against fluoride on DNA damage and apoptosis of human hepatocytes

作　　者：夏涛[1] 杨克敌[1] 褚启龙[1] 张明[1] 刘方[1] 陈学敏[1] 

机构地区：[1]华中科技大学同济医学院环境卫生学教研室,武汉430030

出　　处：《中国公共卫生》2004年第3期270-271,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目(30 371 2 50 )

摘　　要：目的　研究氟、硒对离体人肝细胞DNA损伤及凋亡的作用。方法　体外培养的人肝细胞分别接触一定剂量的氟和 /或硒 12h后 ,检测肝细胞DNA的损伤率、凋亡率和细胞周期构成比。结果　氟组肝细胞DNA损伤率、凋亡率和S期细胞数均明显高于对照组和氟 +硒组 (P <0 0 5) ,硒组DNA损伤率和凋亡小体育分率均高于对照组 ,但无显著性差异 (P >0 0 5)。ObjectiveTo investigate the influence of selenium and fl uoride on DNA damage and apoptosis in human hepatocytes in vitro.MethodsThe DNA damage rate,cellular apoptosis,and cell c ycle in human hepatocytes were measured after hepatocytes were incubated with fl uoride and/or selenium for 12 hours in vitro. ResultsThe DNA damage rate,apoptosis,and cell number in S phase in hepatocytes in the fluoride group were significantly higher than the co ntrol group and the fluoride-exposed and selenium-treated groups (P<0.05).Th e DNA damage rate and apoptosis in hepatocytes in the selenium group were higher than control group,but there was no significant difference between selenium gr oup and control group (P>0.05). ConclusionSome dose of selenium can antagonize DNA damag e and apoptosis in hepatocytes induced by fluoride.

关 键 词：氟 硒 肝细胞 DNA损伤 细胞凋亡 

分 类 号：O612.7[理学—无机化学]