γ-干扰素对染石英尘大鼠肺纤维化的影响  

Effect of γ-interferon on lung silicosis of rats exposed to quartz dust

作　　者：谢妮[1] 陈莹[2] 刘万洋[2] 董静[2] 刘振林[2] 陈杰[2] 

机构地区：[1]中国医科大学公共卫生学院中心实验室,沈阳110001 [2]中国医科大学公共卫生学院尘肺研究室

出　　处：《中国公共卫生》2004年第3期314-315,共2页Chinese Journal of Public Health

基　　金：沈阳市科学技术计划基金 ( 1 0 2 2 0 4 3- 1 - 0 9)

摘　　要：目的　观察γ -干扰素 (IFN -γ)对染石英尘大鼠肺脏器系数及羟脯氨酸含量的影响 ,探讨γ -干扰素对实验性早期矽肺的防治作用。方法　一次性经气管给予雌性Wistar大鼠石英粉尘 2 0mg/只 ,染尘后第 2d开始每天给予γ -干扰素 (1× 10 5 IU/只 ) ,分别在 1个月和 2个月时处死动物。观察大鼠肺脏器系数、肺羟脯氨酸含量变化。结果　给予γ -干扰素 1个月及 2个月时 ,治疗组大鼠肺脏器系数与同期染尘对照组比较有所降低 ,但无显著性差异 ;而治疗组大鼠的肺羟脯氨酸含量均显著低于同期染尘对照组 (P <0 0 5)。结论　γ -干扰素可降低染石英尘大鼠肺羟脯氨酸含量 。ObjectiveTo explore the preventive and therapeutic effec t of γ-interferon to experimental silicosis by studying the effect of γ-interf eron on lung/body coefficient and hydroxyproline in rats exposed to quartz dust.MethodsFemale Wistar rats were exposed to lower dose (20 mg/rat) of quartz dust by intratracheal injection.Beginning from the second day ,the experimenatal rats were treated with γ-interferon (1×10 5IU/rat) daily.R ats were killed after 1 and 2 month.Changes of lung/body coefficient and lung hy droxyproline content were determined.ResultsThe lung/body coefficient showed no significant difference between quartz control groups and treated groups.The lung hydroxyprol ine of treated groups were significantly lower than that of quartz control group s (P<0 05).Conclusionγ-interferon could significanlty reduc e lung hydroxyproline content of quartz exposed rats.This denoted that γ-interf eron may inhibit or delay early silicotic fibrosis.

关 键 词：实验性矽肺 Γ-干扰素 大鼠 肺脏器系数 羟脯氨酸 

分 类 号：R135.2[医药卫生—劳动卫生]