免疫组化检测风湿性心脏瓣膜浸润细胞表型和穿孔素  

Determination of phenotypes of Infiltrating lymphocytes and perform in rheumatic valves by im- munohistochemistry

作　　者：熊丁丁[1] 杨英珍[1] 徐德民[1] 蒋振斌[1] 

机构地区：[1]上海市心血管病研究所,上海医科大学中山医院,200032

出　　处：《中国心血管杂志》1997年第1期15-16,24,共3页Chinese Journal of Cardiovascular Medicine

摘　　要：为了探测细胞介导的细胞毒作用在风湿性心脏瓣膜病(风心病)发病中的意义,我们应用免疫组织化学技术,对9例风心病、6例非风心病瓣膜标本的浸润淋巴细胞表型和穿孔素进行了检测.结果:1.风心瓣膜的浸润细胞中,发现有CD4+细胞5例,CD8+细胞7例,CD16+细胞5例,非风心瓣膜仅1例有少量的CD4+细胞;2.穿孔素仅见于有CD8+和/或CD16+细胞浸润的7例风心瓣膜中.结果初步表明,细胞介导的细胞毒作用可能是风心病发病的主要机理之一,穿孔素则可能是CD8+和CD16+等效应细胞释放的、致瓣膜损伤的主要效应分子.To clarify the role of cell-mediated cytotoxicity (CMC) in pathogenesis of rheumatic heart disease (RHD), we detected the phenotypes cf infiltrating lymphocytes and perforin by im-munohistochemistry in the valves of 9 patients with RHD and 6 patients without RHD. The results were as follows:1. In the valves of 5,7,5,patients with RHD, CD4+.CD8+-CD16 + cells were respectively found, While neither CD8+ nor CD16+ cells were detected in all the nonrheumatic valves and CCD4 + cell was found in only one patient without PHD;2. Perforin was only detected in the valves infiltrated by CD8 + and CD16+ cells of 7 patients with RHD. The data indicate that CMC may be one of the main pathogenesis of RHD, and perforin, as the effector molecule released by effector cell of CMC,may play a critical role in the valve damage occurring in RHD.

关 键 词：免疫组化 检测 风湿性心脏瓣膜病 细胞浸润 细胞表型 穿孔素 淋巴细胞 细胞毒作用 

分 类 号：R541.2[医药卫生—心血管疾病]