甘蔗黑穗病菌拮抗性芽孢杆菌的抗菌作用与伊枯草菌素A的产生有关  

The Antifungal Mechanism of Antagonistic Bacillus subtilus to Ustilago scitaminea is Related to Iturin A Producing

作　　者：张桂英[1] 廖咏梅[1] 张君成[1] 

机构地区：[1]广西大学农学院,南宁市大学路100号530004

出　　处：《广西科学》2004年第3期269-272,共4页Guangxi Sciences

基　　金：广西自然科学基金资助项目 (桂科自 9912 0 15)

摘　　要：选择对甘蔗黑穗病菌有拮抗作用的 5个革兰氏阳性芽孢杆菌拮抗菌株和 3个革兰氏阴性拮抗细菌菌株 ,用 PCR技术 ,扩增抗霉菌枯草杆菌素操纵元中 myc B基因。结果是 ,5个革兰氏阳性芽孢杆菌拮抗菌株均扩增出一条大小为 2 .0 kb左右的特异带 ,革兰氏阳性芽孢杆菌基因组的 PCR产物和伊枯草菌素 A合成酶操纵元中的 Itu B基因的同源性为 97%～ 98% ,说明其抗菌作用机制和 Iturin类群脂肽抗生素的产生有关。 3个革兰氏阴性拮抗细菌中 ,没有扩增到基因 ,其抗菌作用机制有待进一步研究。gram-positive Bacillus strains and 3 gram-negative bacteria strains which are antagonistic to Ustilago scitaminea were selected to be amplified the mycB gene of iturin A operon by PCR.A band of about 2.0 kb appeared among all strains of 5 gram-positive Bacillus.The PCR product is 97%~98% homologous to ItuB gene of iturin A operon.This result shows that the antifungal mechanism of antagonistic Bacillus is related to Iturin group lipopeptin producing.There are no any PCR products appearing among 3 gram-negative bacteria.More research on their antifungal mechanism need to be done in the future.

关 键 词：甘蔗黑穗病菌 拮抗细菌 伊枯草菌素A 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]