夹心免疫PCR方法检测金葡菌肠毒素B  

Establishment of sandwich immuno-PCR technique for detection of SEB

作　　者：宋云扬[1] 吴方晖[1] 张靖[1] 李蕊[1] 王惠芳[1] 李艳军[1] 

机构地区：[1]北京药物化学研究所生化室,北京102205

出　　处：《中国公共卫生》2004年第7期832-833,共2页Chinese Journal of Public Health

摘　　要：目的　建立双抗夹心免疫PCR的检测技术 ,用于检测极微量的抗原。方法　以亲和素作为连接分子 ,连接生物素化抗体和生物素化DNA ,应用于免疫PCR中检测极微量金黄色葡萄球菌肠毒素B(SEB)。结果　双抗夹心免疫PCR的检测技术 ,检测SEB的灵敏度达 0 1ng/L ,与夹心ELISA方法比较灵敏度高 10 3 倍。结论　双抗夹心免疫PCR系统灵敏度高 ,可用于各种微量抗原的检测。Objective To develop sandwich immune-PCR system for monitoring antigen.Methods Avidin was used to bridge the biotinylated antibodies and biotinylated DNA,and to detect antigen by immuno-PCR.Results As little as 0.1 ng/L of SEB could be detected by the sandwich immuno-PCR,which was 10 3 times more sensitive than ELISA parallel control.Conclusion The universal immuno-PCR system can be used to detect ultra-micro antigen.

关 键 词：金葡菌肠毒素B 免疫PCR 戊二醛 生物素 亲和素 

分 类 号：R996.1[医药卫生—毒理学]