不同生长基质对体外培养神经干细胞迁移的影响  

Studies of the effects of different growth substratum on the immigration of neural stem cells

作　　者：扎拉嘎胡[1] 财音青格乐[2] 张锁链[3] 

机构地区：[1]武警医学院细胞生物学与医学遗传学教研室,天津300162 [2]南开大学生命科学院,天津300071 [3]内蒙古大学生命科学院,内蒙古呼和浩特010021

出　　处：《武警医学院学报》2004年第5期354-356,F003,共4页Acta Academiae Medicinae CPAPF

摘　　要：【目的】建立神经干细胞培养的模拟体内条件 ,为寻找更合适的生长基质提供参考。【方法】体外培养获得的大鼠胚胎神经干细胞克隆球种植于用人血浆纤连蛋白和多聚 L 鸟氨酸处理的培养瓶内 ,观察细胞迁移及生长状况 ,分析人血浆纤连蛋白和多聚 L 鸟氨酸对神经干细胞生长迁移的影响。【结果】与未用以上两种基质处理的对照组相比 ,处理组都有细胞迁移 ,但多聚 L 鸟氨酸的效果比人血浆纤连蛋白的效果明显 ,二者协同作用对神经干细胞迁移效果更显著。【结论】生长基质对体外培养神经干细胞的贴壁生长及细胞迁移中具有重要作用。To establish a model condition of culturing neural stem cells,and attempt to provide a ideal dosage of growth substratum in culturing neural stem cells.To culture the rat fetal neural stem cells(rNSCs)in both coated culture bottles with human plasma fibronectin and poly L ornithine,respectiely,and to observe the development and immigration of the rat fetal neural stem cells,and analysis the effects of human plasma fibronectin and poly L ornithine on the growth of neural stem cells.As for the development and migration of rNSCs,poly L ornithine coated group was better than the human plasma fibronectin one,and the fibronectin plus poly L ornithine coated group was the best one.[Conclusion]Growth sunstratum are essential for the development and migration of rNSCs in vitro.

关 键 词：细胞培养 生长基质 大鼠 胚胎神经干细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]