雌激素对大鼠血管平滑肌细胞增殖及基因caveolin-1和Id3α表达的影响  

Effect of estrogen on the proliferation and the gene expression of caveolin-1 and Id3α in rat vascular smooth muscle cells

作　　者：杨发林[1] 贺红[2] 李少燕[1] 唐建华[3] 

机构地区：[1]山东大学齐鲁医院检验科,济南250012 [2]山东大学齐鲁医院心内科,济南250012 [3]山东省血液中心检验科

出　　处：《现代妇产科进展》2004年第5期353-355,共3页Progress in Obstetrics and Gynecology

摘　　要：目的 :探讨雌激素对大鼠血管平滑肌细胞 (VSMCs)增殖及基因囊泡素 1(caveolin 1)和Id3α表达的影响 ,探讨雌激素抑制VSMCs增殖的分子作用机制。方法 :培养Wistar大鼠血管平滑肌细胞 ,经 17β 雌二醇 (17β E2 )处理 2 4h ,用MTT比色法测定细胞增殖水平 ,并提取细胞总RNA ,经甲醛变性凝胶电泳后行Northernblotting分析 ,观察雌激素对血管平滑肌细胞caveolin 1mRNA和Id3α基因mRNA表达的影响。结果 :经 17β E2 处理后的VSMCs增殖受到抑制 ,并呈现浓度依赖趋势 ,Id3α基因mRNA表达和caveolin 1基因mRNA表达均明显增强。结论 :雌激素抑制VSMCs增殖 ,对caveolinObjective:To explore the effect of estrogen on the proliferation and the gene expression of caveolin 1 and Id3α in rat vascular smooth muscle cells (VSMCs).Methods:Wistar rat VSMCs were cultured and pretreated with 17β estradiol(17β E 2) for 24 hours, the proliferation of cultured rat VSMCs was determined by MTT colorimetric method.Total RNA was extracted and Northern blotting was performed to analyze the mRNA expression of caveolin 1 and Id3α.Results:Compared with the cells unpretreated with 17β E 2, the proliferation of VSMCs treated with 17β E 2 (1 100nmol/L) was inhibited in a concentration dependent manner. Northern blotting analysis showed that the expression of caveolin 1 mRNA and Id3α were higher in VSMCs pretreated with 17β E 2 than in VSMCs unpretreated.Conclusion:Estrogen inhibits the proliferation of VSMCs and the up regulation of the gene expression of caveolin 1 and Id3α in vascular wall,which maybe partially indicates the cardiovascular effect of estrogen.

关 键 词：雌激素类 血管平滑肌细胞 DNA结合蛋白质类 细胞质膜微囊蛋白 大鼠 WISTAR 

分 类 号：Q579.1[生物学—生物化学]