东方田鼠精子及冷冻复苏精子体外受精的研究  

The in Vitro Fertilization by Microtus Fortis Spermotoas in Fresh and in Post Frozen-Thawed

作　　者：刘丽均[1] 陆晔[2] 唐一铭[1] 施恩[1] 徐平[1] 

机构地区：[1]中国科学院上海实验动物中心,上海201615 [2]苏州大学生命科学学院,苏州215007

出　　处：《上海交通大学学报（农业科学版）》2004年第3期290-293,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

摘　　要：本文采用Nakagata法,对东方田鼠精子及冷冻复苏后的精子进行体外培养、体外受精,结果显示:东方田鼠精子浓度约为106～108个·μL-1,精子活力冷冻前约为20%～80%,冷冻后约为1%～50%,精子经冷冻复苏后的体外受精率为10%～50%,与冷冻前的体外受精率80%～95%相比,差异显著。This article studies in vitro fertilization of Microtus fortis spe rm otoas in fresh and in post-thawed, using Nakagata's way. The result indicated t hat concentration of fresh sperm is about 106～108·ìL-1, the progressive moti lity of fresh sperm is 20%～80%and post-thawed is 1%～50%respectively. The rate of in vitro fertilization in post-thawed is obviously different, 10%～50 %, comparing with the rate of in vitro fertilization in fresh, 80%～95%.

关 键 词：东方田鼠 精子 冷冻复苏 体外受精 体外培养 体外受精率 实验动物 

分 类 号：S865.1[农业科学—野生动物驯养]