染料木黄酮对哮喘患者核因子-κB表达和肿瘤坏死因子-α分泌的影响  

Effects of genistein on expression of nuclear factor-κB and levels of tumor necrosis factor-α in asthma patients

作　　者：古兴宇[1] 刘晓菊[1] 

机构地区：[1]兰州医学院第一附属医院呼吸科

出　　处：《中国临床药理学与治疗学》2004年第9期1011-1015,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :探讨染料木黄酮对支气管哮喘患者外周血单个核细胞 (PBMCs)核因子 κB(NF κB)表达及肿瘤坏死因子 α(TNF α)分泌的作用。方法 :32例支气管哮喘急性发作期患者及 31例健康对照者的PBMCs ,均分空白对照、地塞米松和染料木黄酮 3组进行研究。NF κB表达由免疫组化染色检测 ,TNF α含量由放射免疫法测定。结果 :哮喘患者PBMCsNF κB胞核染色阳性率及培养上清TNF α含量明显高于健康对照者 (P <0 .0 1) ,且二者呈显著正相关(P <0 .0 1)。染料木黄酮抑制哮喘组NF κB高表达及TNF α高分泌 ,与哮喘空白对照组比较二者均有统计学意义 (P <0 .0 1) ,但其抑制作用弱于地塞米松 ,且哮喘染料木黄酮组PBMCsNF κB胞核染色阳性率与TNF α含量呈显著正相关 (P <0 .0 1)。结论 :哮喘患者NF κB表达增高 ,TNF α分泌增多。染料木黄酮可抑制哮喘患者PBMCsNF κB的高表达及TNF α的高分泌 ,对哮喘可能有潜在的治疗作用。AIM: To investigate the effects of genistein on the expression of nuclear factor-κB (NF-κB) and the levels of tumor necrosis factor-α (TNF-α) in peripheral blood mononuclear cells (PBMCs) of asthma patients. METHODS: PBMCs were isolated from blood samples of 32 asthma patients and 31 healthy persons, which was divided into control groups, dexamethasone-treated groups and genistein-treated groups. The expression of NF-κB was analyzed by immunocytochemical staining. The level of TNF-α was measured by radioimmunoassay. RESULTS: The percentage of NF-κB-positive cells in PBMCs and the level of TNF-α in PBMCs culture supernatants were significantly higher in asthma patients than in healthy persons (all P< 0.01). There was a positive correlation between the percentage of NF-κB-positive cells and the level of TNF-α in asthma patients (P< 0.01). Genistein depressed the expression of NF-κB and the secretion of TNF-α of PBMCs in asthma patients. The percentage of NF-κB-positive cells in PBMCs and the level of TNF-α in PBMCs culture supernatants in asthmatic genistein-treated groups were both significantly lower than those of asthmatic control groups (all P< 0.01), but this depression was not so strong as dexamethasone. There was a positive correlation between the percentage of NF-κB-positive cells and the level of TNF-α in asthmatic genistein-treated groups (P< 0.01). CONCLUSION: There are increases in both expression of NF-κB and the level of TNF-α. Genistein can decrease the expression of NF-κB and the level of TNF-α in asthma patients, so it may has a hidalen effect in treating asthma.

关 键 词：染料木黄酮 支气管哮喘 核因子-KB 肿瘤坏死因子-A 

分 类 号：R977.1[医药卫生—药品]