精制甲型肝炎灭活疫苗的狨猴保护实验  

Protective Effect of Inactivated HA Vaccine on Marmosets

作　　者：练幼辉 胡彩珍[2] 陶林 周彩莲[1] 马波 鲁明 

机构地区：[1]云南沃森生物技术有限公司,昆明650033 [2]广西壮族自治区疾病预防控制中心,南宁530021

出　　处：《中国实验动物学报》2004年第3期180-182,共3页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家高技术研究发展专项经费资助项目(2 0 0 2AA2Z3 3 13 )

摘　　要：目的　检测精制甲型肝炎 (甲肝 )灭活疫苗的保护效果。方法　甲型肝炎病毒 (YN5株 )经Vero细胞培养制成精制甲肝灭活疫苗 ,采用普通狨猴进行疫苗的免疫原性和保护性研究。结果　 7只狨猴均有特异性抗体产生 ,无血清酶活性升高和肝组织学病理改变 ,接种疫苗的狨猴能抵抗甲肝病毒强毒 (Han ZD)株的攻击。但是 ,对照组狨猴均出现血清酶异常升高和肝组织学病理改变。结论　甲肝灭活疫苗 (YN5株 )具有良好的免疫原性和保护效果。Objective To test protective effect of inactivated HA vaccine on marmosets.Methods Inactivated HA vaccine was prepared by culturing the strain YN5 of HA virus in Vero cells. Its immunogenicity and protection was studied in marmosets(Saguinus). Results Specific anti-HAV antibodies were induced in 7 common marmosets, and no elevation of serum enzyme activity or pathological exchange of liver tissue were observed. The vaccine protected marmosets against the intravenous challenge with virulent HAV(Han-ZD). But the elevation of serum enzyme activity and pathological exchange of liver tissue were showed in all the control marmoets. Conclusion The inactivated HA vaccine (YN5) had good immunogenicity and protective effect.

关 键 词：甲型肝炎 灭活疫苗 狨猴 免疫保护 免疫原性 

分 类 号：R512.61[医药卫生—内科学]