新型CKLF1对家兔红系造血祖细胞增殖分化的影响  

THE ROLE OF CKLF1 ON THE PROLIFERATION ANDDIFFERENTIATION OF RABBIT ERYTHROID PROGENITORS

作　　者：岳黎敏[1] 唐军民[2] 苏安英[1] 唐岩[2] 王金华[3] 汪涛[4] 文宗曜[3] 

机构地区：[1]河北工程学院医学部组织学胚胎学教研室,河北056029 [2]北京大学基础医学院人体解剖学与组织胚胎学系,北京100083 [3]北京大学基础医学院生物物理学教研室,北京100083 [4]天津中医学院组织学胚胎学教研室,天津300193

出　　处：《解剖学报》2004年第4期382-385,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金重点课题资助项目 ( 3 983 0 110 )

摘　　要：目的　探讨新型趋化因子CKLF1对家兔骨髓单个核细胞形成红系集落的影响 ,并初步探讨其作用机制。　方法　采用尼龙毛去除骨髓基质细胞法、甲基纤维素半固体培养法检测新型趋化因子CKLF1对家兔红系形成的影响。　结果　新型趋化因子CKLF1具有促进家兔骨髓单个核细胞形成红系克隆的作用 ,并且该作用与CK LF1的浓度呈正相关 ;去除骨髓基质细胞后 ,CKLF1对红系克隆的形成无明显影响 ;另外 ,CKLF1刺激制备的基质细胞上清也具有促进红系克隆形成的作用。　结论　新型趋化因子CKLF1可能是通过调控骨髓基质细胞而间接促进红系克隆的形成。Objective To study the effect of CKLF1 on the formation of erythroid colonies. Methods Semisolid-culture and depletion of bone marrow stromal cell by nylon wool method were adopted to examine the effect of CKLF1 on the formation of erythroid colonies. Results 1. CKLF1 can promote the formation of erythroid colonies from rabbit bone marrow mononuclear cells and the number of the colonies is positively correlated with the concentration of CKLF1; 2. CKLF1 has no obvious effect on the formation of erythroid colonies after depletion of bone marrow stromal cell; 3. The supernatant, which was collected from stromal cells cultured with CKLF1, can also promote the formation of erythroid colonies.Conclusion The results indicate that CKLF1 may promote the erythroid colonies formation by regulating the stromal cells.

关 键 词：趋化因子CKLF1 红系克隆 骨髓 基质细胞 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]