大鼠肝再生过程中碱性磷酸酶活性变化及其超微细胞化学研究  

THE ACTIVITY CHANGES OF ALKALINE PHOSPHATASE AND ITS ULTRASTRUCTUAL LOCALIZATION DURING LIVER REGENERATION IN RATS

作　　者：李金亭[1] 王俊丽[1] 傅山岗[1] 李晓英[1] 马克学[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,新乡453002

出　　处：《解剖学报》2004年第4期392-395,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 ( 3 9670 3 72 ) ;河南省自然科学资金资助项目 ( 964 0 2 180 0 )

摘　　要：目的　研究 2 / 3肝切除后大鼠肝再生过程中碱性磷酸酶 (AKP)活性的变化及其细胞定位。　方法　利用比色法对AKP活性进行定量分析 ;用酶的原位复性电泳技术分析再生肝中AKP的种类和活性变化 ;用电镜细胞化学方法研究酶定位。　结果　在肝部分切除后的肝再生期间 ,AKP出现两个活性高峰 (16h和 19h) ,在每个活性高峰后 ,AKP均有显著下降 ;获得 3种肝型AKP同工酶 (14 0、16 0和 180kD) ,其中 180kD的AKP只在肝再生过程中出现 ;随着肝再生的进展 ,AKP活性出现在细胞的不同部位。　结论　AKP在肝再生过程中起重要作用 ,可能参与细胞代谢、物质转运、DNA合成和细胞分化。Objective To study the changes of AKP activity and its cytochemistry position during liver regeneration following partial hepatectomy. Methods Using colorimetry to analyze AKP activity; using non-denatured electrophoresis technique to analyze the kinds and activity of AKP; using electron microscope cytochemistry to analyze its position. Results During liver regeneration, AKP activity had two peaks at 16*!h and 19*!h and followed by decline dramatically; Three hepatic-derived AKP isoenzymes were detected, and 180*!kD AKP appeared only in liver regeneration; AKP appeared in different part with the process of liver regeneraion.Conclusion AKP plays an important role in liver regeneration and it may involve in cell metabolism, material transportation, DNA synthesis and cell differentation.

关 键 词：肝再生 碱性磷酸酶 细胞化学定位 酶原位复性电泳 

分 类 号：Q26[生物学—细胞生物学] Q55