灯盏花素对兔血管平滑肌细胞增殖的影响  

Effect of Breviscapine on the Proliferation of Rabbit Vascular Smooth Muscle Cells

作　　者：庞荣清[1] 潘兴华[1] 龙沛然[2] 覃敏[2] 吴亚玲[3] 

机构地区：[1]成都军区昆明总医院医学实验科 [2]成都军区军事医学研究所电镜室,云南省昆明市650032 [3]成都军区昆明总医院中医科

出　　处：《中国动脉硬化杂志》2004年第4期395-398,共4页Chinese Journal of Arteriosclerosis

基　　金：云南省自然科学青年基金 ( 2 0 0 1C0 2 9Q)资助

摘　　要：观察灯盏花素对兔血管平滑肌细胞生长增殖、细胞超微结构及其细胞周期和核因子κB活性的影响 ,以探讨其药理机制。胎牛血清刺激体外培养的兔血管平滑肌细胞使其快速增殖生长 ,加入不同浓度的灯盏花素作用一定时间后 ,通过MTT法测定灯盏花素对血管平滑肌细胞生长的抑制率 ,流式细胞技术测定细胞周期和核因子κB活性以及用透射电镜观察细胞超微结构的变化。结果发现 ,灯盏花素能明显抑制胎牛血清刺激的血管平滑肌细胞增殖 (IC5 0为 17.9mg L) ,并改变细胞的超微结构 ,包括使细胞电子密度增大 ,部分胞膜断裂 ,粗面内质网和高尔基体呈空泡状扩张 ,部分线粒体呈凝固性变化 ,嵴减少或消失 ,或呈凝固性坏死。灯盏花素还以浓度依赖方式减少S期细胞 ,阻滞细胞于G0 G1 期 ,并下调核因子κB活性。结果提示 ,灯盏花素能显著抑制血管平滑肌细胞的增殖生长 ,可能具有抗动脉粥样硬化的作用 ,其作用机理可能部分是通过调控血管平滑肌细胞核因子κB活性来实现的。Aim To investigate the efffect of breviscapine on the proliferation, cell ultrastructure, cell cycle and nuclear factor-κB (NF-κB) activity of rabbit vascular smooth muscle cell (VSMC) in order to find out the mechanism of its action. Methods By techniques of cell culture in vitro, VSMC stimulated by fetal bovine serum were treated by breviscapine in different concentration for a certain period, then the inhibitory ratio of cell proliferation was measured by MTT, cell cycle distribution and NF-κB activity of the cell were measured by Flow-Cytometry respectively, and the cell ultrastructure was observed by transmission electron microscopy (TEM). Results Breviscapine could obviously inhibit the proliferation of VSMC stimulated by fetal bovine serum (IC50 value was 17.9 mg/L), and change the cell ultrastructure, which included that electron density increased, a part of cytomembrane lysised, endoplasmic reticulum and Golgi apparatus showed vacuolar degeneration, some mitochondrion showed coagulation and mitochondrial crista disappeared or showed coagulation. Breviscapine could also decrease S phase cells, increase G 0/G 1 phase cells and down-regulate NF-κB activity of VSMC by concentration-dependent fashion, and the results were significant compared with the control (P<0.01). Conclusion Breviscapine could obviously inhibit the proliferation of VSMC and may prevent atherosclerosis, which mechanism may be realized partly by regulating NF-κB activity of VSMC.

关 键 词：细胞生物学 灯盏花素对血管平滑肌细胞增殖的影响 细胞培养 灯盏花素 血管平滑肌细胞 细胞周期 核因子ΚB 

分 类 号：Q2[生物学—细胞生物学]