脂多糖诱导的血管外膜炎症对兔股动脉内膜增生的影响及机制  

Influence of Lipopolysaccharide-induced Adventitial Inflammation on Intimal Proliferation of Rabbit Iliac Artery and Its Mechanism

作　　者：胡鹏[1] 李建军[1] 陈莉[1] 王晶[1] 

机构地区：[1]武汉大学人民医院心内科,湖北省武汉市430060

出　　处：《中国动脉硬化杂志》2004年第3期330-332,共3页Chinese Journal of Arteriosclerosis

摘　　要：通过用脂多糖诱导兔股动脉外膜炎症,来观察其对内膜增生的影响,并用免疫组织化学法观察股动脉壁核因子κB p65的表达情况,从而探讨外膜炎症对内膜增生影响的分子机制。高脂饮食饲养新西兰大耳白兔24只,两侧股动脉按实验要求分为分为脂多糖组(n=24)和对照组(n=24)。制备用脂多糖诱导的外膜炎症模型,于术后0天(n=8)、3天(n=8)和2周(n=8)处死动物取得股动脉标本,HE染色观察形态学变化和内膜增生情况,同时用免疫组织化学方法镜下观察核因子κB p65在动脉壁的表达。结果发现3天和2周时,脂多糖组血管外膜和内膜面炎症细胞明显增多。在3天时,脂多糖组血管内皮细胞和外膜细胞在胞浆中出现核因子κB p65阳性表达。计算机图象分析发现,2周时脂多糖组内膜面积(0.93±0.14mm2)和对照组(0.75±0.15mm2)相比有明显差异(P<0.05)。结果提示脂多糖诱导的血管外膜炎症可导致兔股动脉内膜增生,而在内膜增生之前伴随着血管内皮细胞和外膜细胞核因子κBp65的阳性表达,外膜炎症可能是通过激活上述细胞中的核因子κB从而启动炎性细胞因子的转录导致内膜增生。Aim To investigate the influence of lipopolysaccharide (LPS)-induced adventitial inflammation on artery in timal proliferation and its mechanism. Methods 24 New Zealand rabbits were fed with high cholesterol diet for 2 weeks, two sides of iliac artery were randomly divided into two groups: LPS group ( n = 24) and control group ( n = 24). A nonconstrictive polyethylene cuff was placed loosely around the iliac artery of New Zealand rabbits, between the artery and the cuff, and a sterilized cotton thread was placed, which was previously soaked overnight in 6 mg/L LPS in saline. Animals were killed at intervals of 0 day ( n = 8) ,3 days { n = 8), 2 weeks ( n = 8) after surgery. The morphology of the artery and the immunochemistory expression of nuclear factor (NF) κB p65 in the artery was investigated. Results In LPS group, 3 days after surgery, inflammation cells migrated into artery from both the luminal and adventitial side, and 2 weeks after surgery, inflammation cells could only be seen in adventitia; NFκB p65 was expressed in endothelium and adventitial cell in LPS group 3 days after surgery; 2 weeks after surgery, LPS group have increasd intimal area (0.93 ± 0.14 mm2), compared with control group(0.75 ± 0.15 mm2) (P < 0.05). Conclusions LPS-induced adventitial inflammation contributes to intimal proliferation, and the activation of NFκB in endothelium and adventital cells may be the mechanism of it.

关 键 词：内科学 外膜炎症对内膜增生的影响 免疫组织化学法 股动脉 核因子ΚB 兔 

分 类 号：R543[医药卫生—心血管疾病]