Vaccination with pcDNA3-15/60 Naked DNA Encoding the Surface Protein of Sporozoites in Cryptosporidium parvum  

作　　者：HEHong-xuan ZHANGXi-chen YINJi-gang LIJian-hua YANGJu 

机构地区：[1]DepartmentofVeterinaryPreventiveMedicine,FacultyofAnimalScience,theQuartermasteruniversityofChinesePeople＇sLiberationArmy,Changchun130062,P.R.China

出　　处：《Agricultural Sciences in China》2004年第8期634-640,共7页中国农业科学（英文版）

基　　金：supported by Natural Science Foundation of Jilin Province for Outstanding Young scientists Award,China(2002).

摘　　要：The CP15/60 gene encoding the CP15/60 surface protein of sporozoites in Cryptosporidiumparvum was obtained by PCR so as to research the nucleic vaccine against C.parvum. Theeukaryotic expressing vector pcDNA3-15/60 was constructed by inserting CP15/60 gene intopcDNA3 (+) in XhoⅠand EcoRⅠ. A vaccination protocol was the adult pregnant goatsinoculated intranasally with the pcDNA3-15/60 plasmid and their offspring were infectedwith C.parvum oocysts. The results showed that the pcDNA3-15/60 plasmid can induce theimmune response of goats and the vaccinated goats can transfer the immunity to offspringconferring protection against C.parvum infection. These suggested that the recombinantplasmid could be a DNA vaccine candidate.The CP15/60 gene encoding the CP15/60 surface protein of sporozoites in Cryptosporidiumparvum was obtained by PCR so as to research the nucleic vaccine against C.parvum. Theeukaryotic expressing vector pcDNA3-15/60 was constructed by inserting CP15/60 gene intopcDNA3 (+) in XhoⅠand EcoRⅠ. A vaccination protocol was the adult pregnant goatsinoculated intranasally with the pcDNA3-15/60 plasmid and their offspring were infectedwith C.parvum oocysts. The results showed that the pcDNA3-15/60 plasmid can induce theimmune response of goats and the vaccinated goats can transfer the immunity to offspringconferring protection against C.parvum infection. These suggested that the recombinantplasmid could be a DNA vaccine candidate.

关 键 词：Cryptosporidium parvum (C.parvum) CP15/60 Nucleic acid vaccine Nasal immunization 

分 类 号：R392.11[医药卫生—免疫学] S852.52[医药卫生—基础医学]