HPLC法测定胃肠舒胶囊中葛根素、橙皮苷、丁香酚、和厚朴酚、厚朴酚的含量  

Determination of Puerarin, Hesperidin, Eugenol, Honokiol and Magnolol in Weichangshu Capsule by HPLC

作　　者：谢友良[1] 曾宝[1] 吴燕红[1] 李俊哲[1] 曾惠芳[1] 叶其馨[1] 苏子仁[1] 

机构地区：[1]广州中医药大学,广州510405

出　　处：《中药新药与临床药理》2004年第5期345-347,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立复方制剂胃肠舒胶囊中多种有效成分的含量测定方法。方法采用HPLC法,PhenomenexlunaC18(2)色谱柱,甲醇-水梯度洗脱分离制剂中葛根素、橙皮苷、丁香酚、和厚朴酚、厚朴酚等有效成分,并对其进行含量测定,流速1mL·min-1,检测波长283nm,柱温25℃。结果葛根素、橙皮苷、丁香酚、和厚朴酚、厚朴酚的线性范围分别为0.125～2.000μg、0.031～1.020μg、0.117～2.340μg、0.142～2.280μg、0.117～1.880μg;平均回收率分别为100.43%、99.44%、100.37%、100.01%、99.64%。结论该测定方法简便可行、重复性好,可用于胃肠舒胶囊中各有效成分的含量测定。Objective To establish a method for the determination of effective components in Weichangshu Capsule.Methods Puerarin, hesperidin, eugenol, honokiol and magnolol were isolated by gradual elution with methanol-water, and their contents were determined by HPLC. The chromatographic conditions were: Phenomenex luna C18 (2)column, flowing rate being 1mL·min-1, detecting wavelength at 283 nm and the column temperature being 25 ℃. Results A good linearity of puerarin, hesperidin, eugenol, honokiol and magnolol was in the range of 0.125～2.000 μg, 0.031～1.020 μg, 0.117～2.340 μg, 0.142～2.280 μg and 0.117～1.880μg respectively;the average recovery was 100.43 %, 99.44 %, 100.37 %, 100.01 %and 99.64 %respectively. Conclusion The method is simple, feasible and reproducible and can be used for the quality control of Weichangshu Capsule.

关 键 词：胃肠舒胶囊 葛根素 橙皮苷 丁香酚 和厚朴酚 厚朴酚 含量测定 

分 类 号：R284.1[医药卫生—中药学]