SARS-CoVS基因表达及纯化  

Expression and purification S protein of SARS-CoV

作　　者：柴明青[1] 张东华[2] 陆志檬[2] 谢幼华[3] 沈忠明[1] 

机构地区：[1]上海大学生命科学学院,上海200436 [2]上海第二医科大学,上海200025 [3]中国科学院上海生命科学研究院,上海200031

出　　处：《上海师范大学学报（自然科学版）》2004年第3期72-76,共5页Journal of Shanghai Normal University(Natural Sciences)

摘　　要：严重急性呼吸道综合症是由一种新的冠状病毒SARS-CoV引起的.作者通过PCR扩增得到了S蛋白的6个编码片段,并利用表达载体pET28a(+)在E.coli BL21中进行了原核表达.通过亲和层析纯化了包含大部分ACE2结合区域的S蛋白片段(S4).ELISA分析结果表明S4与SARS病人恢复期血清具有良好的反应能力.A distinct coronavirus (SARS-CoV) has been identified as the etiological agent of Severe Acute Respiratory Syndrome (SARS). We obtained six coding fragments of the SARS-CoV S gene by PCR and synthesized them in Escherichia cob BL21. The S4 protein which contains a large part of the ACE - 2 binding region was purified by nickel - affinity chromatography. ELISA showed that the S4 protein had a good reactivity with the sear from a SARS patient.

关 键 词：SARS S蛋白 表达纯化 血清检测 

分 类 号：Q786[生物学—分子生物学]