维生素E对慢性镉染毒小鼠肾近端小管细胞损伤的保护作用  

Protective Effects of VE on Renal Proximal Tubular Cells of Mice With Chronical Cadmium Poisoning

作　　者：苏敏[1] 黄悦[1] 姜俸蓉[1] 许庭良[1] 

机构地区：[1]贵阳医学院组织胚胎学教研室,贵州贵阳550004

出　　处：《环境与健康杂志》2004年第5期298-300,共3页Journal of Environment and Health

基　　金：贵州省科技厅基金资助项目(E98-2)

摘　　要：目的探讨维生素EVE对慢性染镉小鼠肾近端小管细胞损伤的保护作用。方法75只昆明种小鼠随机分为对照组、镉组和VE组,每组25只。镉组:每周2次经皮下注射氯化镉2mg/kg,给药体积5ml/kg;VE组:同时按10mg/(kg·d)给予VE;对照组:经皮下注射等量生理盐水。3个月后,用光镜、透射电镜、免疫组化细胞凋亡染色(Tunel法)观察经VE处理的慢性染镉小鼠肾近端小管上皮细胞细微结构的改变,并作形态计量分析;观察细胞凋亡情况,对凋亡细胞进行计数。结果经VE处理的慢性染镉小鼠肾近端小管上皮细胞形态结构与正常对照组接近,与镉组比较有明显不同;上皮细胞核的平均截面周长、平均截面积、平均表面积、平均体积和体积密度等形态参数值与镉组比较差异也有显著性(P<0.05);与对照组相比,镉组细胞凋亡染色阳性反应细胞数量明显增多(P<0.01),而VE组数量无明显变化(P>0.05)。结论VE对慢性染镉引起的小鼠肾近端小管上皮细胞损害有保护作用。Objective To study protective effects of vitamin E(VE) on renal proximal tubular cells of mice treated with cadmium chronically. Methods 75 Kunming mice were divided into 3 groups randomly, cadmium group, VE group, control group. The mice in cadmium group were treated with cadmium in 2 mg/kg by subcutaneously injected, twice per week, in VE group they were treated with VE in 10 mg/(kg·d) additionally, in control group the animals were treated with saline only. 3 months later the finestuctural changes of the renal proximal tubule cells were observed by electron microscopy and immunohistochemistry(Tunel method). The ultrastuctures of nuclei were revealed with stereological analysis. The apoptotic cells were counted with image analysis. Results The structure of the renal proximal tubule cells of mice in the VE group was similar to those in the control group, but it had significant changed compared with those of the cadmium group. Compared with the control group, the nuclear important morphological parameters of VE group increased significantly (P<0.05). The numbers of apoptotic cells of VE group were less than those of cadmium group(P<0.01). Conclusion VE could prevent renal proximal tubular cells from damage induced by cadmium.

关 键 词：维生素E 镉 肾近端小管 细胞凋亡 形态计量分析 

分 类 号：R114[医药卫生—卫生毒理学]