Caco-2细胞模型建立及其在纳米脂质体包裹胰岛素体外吸收研究中的应用  

Establishment of Caco-2 Cell Model and the Application on the Transport Study in Vitro of Nano-liposome Coated Insulin

作　　者：李正荣[1] 王琰[1] 潘飞燕[1] 王文[1] 郭建新[2] 平其能[2] 李朝军[1] 

机构地区：[1]南京师范大学江苏省分子医学生物技术重点实验室,江苏南京210097 [2]中国药科大学药剂教研室,江苏南京21000

出　　处：《南京师大学报（自然科学版）》2004年第3期88-91,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金重点资助项目(39930200).

摘　　要：　为建立Caco 2细胞模型,并用于纳米脂质体包裹胰岛素的体外吸收特性研究.通过Caco 2细胞培养于Transwell上,培养20余天,使其生长分化成单层小肠上皮样细胞,然后以单层细胞电阻,电镜观察小肠上皮结构的形成,碱性磷酸酶反应测定单层细胞极性以及对葡聚糖的通透特性等指标对该模型进行评估,并运用Caco 2细胞模型,对胰岛素和纳米脂质体胰岛素进行通透特性研究.结果表明,Caco 2细胞形成刷状缘及紧密连接结构,碱性磷酸酶活性集中在刷状缘侧,单层细胞电阻达到300～500Ω/cm2,类似小肠上皮.纳米脂质体包裹后,胰岛素的通透量显著增加.说明纳米脂质体包裹可提高胰岛素的通透性,可以作为胰岛素口服用药的有效方法.To establish Caco-2 cell model and to use it evaluate the permeating characteristics of peroral nano-liposome coated insulin. Caco-2 cells were cultured on transwell for more than 20 days to get an epithelium like monolayer. Parameters of the cell model were assayed such as transepithelial electrical resistance, ultrastructure, the distribution of alkaline phosphatase activity and the permeability of Dextran-Rhodamine across the monolayer. The transport characteristics of insulin and insulin encapsuled in nano-liposome was also examined using the established cell model. Results: Like intestinal epithelial cells, Caco-2 cells formed brush borders and tight junction between adjacent cells. Alkaline phosphatase activity was detected on the side of the brush border. Transepithelial electrical resistance arrived 300～500?Ω/cm^2. Dextran-Rhodamine cannot enter or across the monolayer of the Caco-2 cells. Nanoliposome encapsulation can increase the permeability of insulin across monolayer of Caco-2 cell. Conclusion: Using the established Caco-2 cell model, we found that nanoliposome encapsulation is a good method to promote bioavailabilities of peroral insulin.

关 键 词：口服胰岛素 CACO-2细胞模型 纳米脂质体 

分 类 号：Q502[生物学—生物化学] Q578