家蚕浓核病毒(镇江)株主要结构蛋白基因的克隆及表达  

Cloning of the Major Structural Protein Gene of Densovirus (Zhenjiang Isolate) in Silkworm,Bombyx mori,and Its Expression in Escherichia coli

作　　者：许光治[1] 张志芳[1] 覃光星[1] 黄可威[1] 郭锡杰[1] 

机构地区：[1]中国农业科学院蚕业研究所农业部家蚕生物技术重点开放实验室,江苏镇江212018

出　　处：《病毒学报》2004年第3期279-282,共4页Chinese Journal of Virology

摘　　要：The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain （BmDNV-ZJ）,sequenced and expressed in Escherichia coli.The gene consists of 1500 nucleotides and is predicted to code a protein of 499 amino acids.An identity of 98.1% in nucleotide sequence and 98.6% of the predicted amino acid sequence are shared with its homologue,Bombyx mori densovirus （Yamanashi isolate） VD1-ORF2,respectively.The gene was inserted into expression plasmid vector pET-28a and expressed in Escherichia coli BL21（DE3） strain under the inducible promoter Lac.The expressed 53kD product could be detected by Western blotting with viral antiserum.It suggests that the gene we cloned is a structural protein gene.The major structural protein gene was cloned from Bombyx mori densovirus,Zhenjiang strain (BmDNV-ZJ),sequenced and expressed in Escherichia coli.The gene consists of 1500 nucleotides and is predicted to code a protein of 499 amino acids.An identity of 98.1% in nucleotide sequence and 98.6% of the predicted amino acid sequence are shared with its homologue,Bombyx mori densovirus (Yamanashi isolate) VD_1-ORF_2,respectively.The gene was inserted into expression plasmid vector pET-28a and expressed in Escherichia coli BL21(DE3) strain under the inducible promoter Lac.The expressed 53kD product could be detected by Western blotting with viral antiserum.It suggests that the gene we cloned is a structural protein gene.

关 键 词：家蚕 浓核病毒 结构蛋白 基因 克隆 表达 

分 类 号：S884.5[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]