鸡肌肉组织中氯霉素残留ELISA检测方法的研究  

Development of an enzyme linked immunosorbent assay for CAP in chicken muscle tissues by the polyclonal antibody

作　　者：魏书林[1] 史为民[1] 彭莉[2] 沈建忠[1] 原蕾[1] 崔伟[1] 

机构地区：[1]中国农业大学动物医学院,北京海淀100094 [2]四川省兽药监察所,四川成都610041

出　　处：《中国兽医杂志》2004年第9期7-9,共3页Chinese Journal of Veterinary Medicine

基　　金：国家"十五"科技攻关资助课题 ( 2 0 0 2 BA5 14 A14 ) ;国家自然科学基金项目 ( 3 0 3 2 5 0 3 2

摘　　要：本文利用活化酯法合成了氯霉素抗原 ,作为免疫原免疫新西兰大耳白兔得到氯霉素的多克隆抗体 ,建立了氯霉素间接竞争酶联免疫检测方法 ,进行了药物交叉反应性试验 ,并对鸡肌肉进行添加回收试验。结果显示抗体效价可达 1∶ 6 4 0 0 0 0 ,IC50 为 1.3ng/ ml,最低检测限达到 0 .0 5 ng/ ml,线性检测范围为 0 .1～ 36 .4 5 ng/ ml。在 0 .5、1、2 .5、5 ng/ g浓度水平添加到鸡肌肉组织中 ,测得回收率为 5 5 .4 %～ 119.0 % ,变异系数为 4 .3%～ 10 .4 %。该 EL ISA方法快速、灵敏、方便 ,满足了氯霉素残留检测的要求。A sensitive and specific ELISA for detection of CAP was established by anti-chloramphenicol polyantibody(PcAb). In the ciELISA the best concentration of coating antigen (CAP-OVA) was 1.7 μg/ml and the titer of anti-serum was 1∶640 000. The optional working dilution of the PcAb was 1∶20 000 after purification. The linear detecting range of calibration curves to detect CAP was 0.1～36.45 ng/ml, and the limit of detection was 0.05 ng/ml. The recoveries ranged from 55.4% to (119.0%) for detecting CAP residues in chicken muscle tissues,and the coefficients of variation was 4.3%～(10.4%).

关 键 词：鸡 肌肉组织 氯霉素残留 ELISA检测方法 多克隆抗体 

分 类 号：S858.31[农业科学—临床兽医学] S859.84[农业科学—兽医学]