中药复方对尾吊大鼠心肌ATP酶、SDH和能荷值的影响  

Effects of Chinese Herb Compound on Myocardial SDH,ATP-ase and Energy Reserves in Tail-suspended Rats.

作　　者：王宝珍[1] 李勇枝[1] 辛冰牧[1] 范全春[1] 白桂娥[1] 

机构地区：[1]航天医学工程研究所,北京100094

出　　处：《航天医学与医学工程》2004年第5期326-329,共4页Space Medicine & Medical Engineering

基　　金：国家载人航天工程资助项目

摘　　要：目的观察中药复方对短期模拟失重 (尾吊 )大鼠心肌ATP酶、SDH和能荷值的影响。方法大鼠分对照组、尾吊组和中药组 (中药 +尾吊 ) ,每组 1 0只。尾吊 5d后 ,3组大鼠同时处死 ,取心肌测ATP酶和SDH ,酶不同染色处理并定量测其光密度 (OD) ,测心肌能荷值定量表示能量储备。结果尾吊组心肌细胞SDH染色比中药、对照组增强 ,OD值升高 ,ATP酶明显增强和OD值显著升高 ,心肌能荷值减少 ,其顺序为尾吊组 >对照组 >中药组。结论中药组在尾吊时由于中药复方发挥了镇静、安神、活血化瘀等药理作用 ,使大鼠心肌SDH活性减弱 ,ATP酶活性明显降低 。ObjectiveTo investgate effects of Chinese herb compound on myocardial SDH,ATP-ase and energy reserves in tail-suspended rats. Method Male SD rats were randomly divided into three groups(n=10 each): (A) normal control group; (B)tail-suspended group; (C) tail-suspended + Chinese Medical herb compound group.Rats in group B and C were tail-suspended(-30°) for 5 d to simulate weightlessness. All rats B were dicapitated aftar the experiment. Myocardial SDH(method of Anderson N-BT),ATP-ase(method of Wachstein and Meisel) and energy reserves (method of HPLC)were examined. Result Compared with rats in groups A and C , SDH activity increased, SDH staining deepened,value of OD SDH enhanced,ATP-ase activity and OD value increased and enhanced signeficently(P<0.01)but value of myocardial energy reserves decreased in group B rats. Conclusion Tail-suspension strengthens compensatory oxidation metabolism of cardiac muscle and activats SDH and ATP-ase,at the same time decrease,myocardial energy reserves. Normal are maintained the low level of metabolism by the medical herb compound used which shows a protecting effects through mitigation,tranguilization and replenishment of Qi.

关 键 词：失重模拟 尾吊 中药学 复方 心肌 ATP酶 琥珀酸脱氢酶 能荷值 

分 类 号：R852.22[医药卫生—航空、航天与航海医学] R856.2[医药卫生—临床医学]