ROK在14d尾部悬吊大鼠股动脉收缩中的作用  

Role of ROK in Contraction of Rat Femoral Arteries after 14 d Tail Suspension.

作　　者：李志利[1] 袁明[1] 姜世忠[1] 汪德生[1] 王惠娟[1] 

机构地区：[1]航天医学工程研究所,北京100094

出　　处：《航天医学与医学工程》2004年第5期330-333,共4页Space Medicine & Medical Engineering

基　　金：中国载人航天工程基金项目

摘　　要：目的研究Rho连接激酶 (Rhoassociatedkinase ,ROK)在 1 4d尾部悬吊大鼠股动脉收缩中的作用。方法采用 1 4d尾部悬吊大鼠 ( - 30°)模拟失重效应 ,Wistar大鼠被随机分成 2组 :自由活动组 (对照组 ,CON)和悬吊组 (TS1 4d) ,利用离体血管环灌流技术检测ROK抑制剂Y 2 7632对KCI、苯肾上腺素(PE)和U 4661 9诱导的大鼠股动脉收缩反应性的影响。结果与对照组相比 ,悬吊组股动脉对 68mmol/LKCl和 1 0 - 5 mol/LPE的收缩反应性显著下降 ,而对 1 0 - 6 mol/LU 4661 9诱导的收缩反应下降不显著 ;在 68mmol/LKCI诱导的收缩中 ,Y 2 7632对收缩的抑制效应随剂量的增加而增强且在悬吊组更强 ,在Y 2 7632由 1 0 - 6 mol/L增加到 1 0 - 5 mol/L时 ,悬吊组股动脉的紧张性收缩 (toniccontraction)几乎被完全抑制 ,在PE和U 4661 9诱导的收缩中 ,Y 2 7632对收缩的抑制效应在悬吊组较强 ;在Y 2 7632作用下 ,悬吊组收缩力下降的幅值较高 ,但下降的幅值在KCI和PE诱导的收缩中没有显著性差异。结论 1 4d尾部悬吊增强了大鼠股动脉RhoA ROK通路的活性 。Objective To determine the role of Rho associated kinase (ROK) in contraction of rat femoral arteries after 14 d tail suspension. Method Male Wistar rats were randomly divided into control group (CON) and 14 d -30° tail suspension group (TS14d). Analysis was performed on the contractile responses of perfused femoral arterial rings from both TS14d and CON rats to KCI, phenylephrine (PE), and U-46619 in the presence of Y-27632. Result Arterial rings from TS14d rats displayed a reduced contractile response to KCI and PE but not significantly to U-46619. In the response to KCI, Y-27632 caused a concentration-dependent relaxation and significantly larger decrease of contraction in tissues from TS14d rats. Y-27632 nearly abolished the tonic component of KCI -induced contraction when its dose was increased from 10 -6 mol/L to 10 -5 mol/L. It had also an inhibitive effect on the PE and U-46619-induced contraction and caused significantly larger decrease in U-46619 but not in KCI or PE induced contraction in tissues from TS14d rats. Conclusion ROK activity may be enhanced and play a compensational role in rat femoral arteries after TS14d.

关 键 词：连接激酶 失重模拟 尾吊 股动脉 血管反应性 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]