二至天癸方对小鼠卵细胞质量影响的实验研究  

Experimental Study on Effect of Erzhi Tiangui Recipe on Quality of Oocyte in Mice

作　　者：连方[1] 孙振高[1] 张建伟[2] 张宁[2] 刘延荷[1] 穆琳[2] 张鹏[2] 

机构地区：[1]山东中医药大学附属医院,济南250011 [2]山东中医药大学

出　　处：《中国中西医结合杂志》2004年第7期625-627,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助课题 (No .90 2 0 90 58)

摘　　要：目的观察二至天癸方在体外受精 胚胎移植过程中对卵细胞质量的影响。方法将 80只小鼠随机分为 4组 :结合组〔给二至天癸方加人绝经期促性腺激素 (HMG)〕、中药组 (给二至天癸方 )、激素组 (给HMG)、对照组 (给生理盐水 ) ,并进行排卵和卵裂试验 ,观察中药对卵细胞质量的影响。结果结合组与激素组在促排卵数量上差异无显著性 ,但在卵细胞形态学评分及受精率、卵裂率中比较差异均有显著性 (P <0 0 5 ) ,以结合组为优。结论二至天癸方可与西药发挥协同作用 ,并能提高卵细胞质量。ObjectiveTo observe the effect of Erzhi Tiangui recipe (ETR) on quality of oocyte in the process of external fertilization and embryo-transplantation. MethodsEighty mice were randomly divided into 4 groups, Group A treated with ETR plus human menopausal gonadotropin (HMG), Group B with ETR, Group C with HMG and Group D with normal saline. Ovulation test and cleavage test were conducted to observe the effect of treatment on quality of oocytes. ResultsThe difference on ovulation number between Group A and C was insignificant, but the difference in comparison between the two groups was significant in aspects of oocyte morphological scoring, fertilization rate and cleavage rate (P<0 05).ConclusionETR could play its effect synergistically with Western medicine, and raise the quality of oocytes.

关 键 词：二至天癸方 小鼠 卵细胞 胚胎移植 体外受精 

分 类 号：R285[医药卫生—中药学]