巯基物质对大鼠颅脑外伤后的胃细胞保护作用  

Gastric cytoprotection by sulfhydryl compounds:stress-relation mucosal damage in rats with brain trauma

作　　者：周婷婷[1] 张峻[2] 杨昭徐[2] 崔培林[2] 

机构地区：[1]首都医科大学附属北京朝阳医院综合科,100020 [2]首都医科大学附属北京天坛医院消化内科

出　　处：《北京医学》2004年第4期232-235,共4页Beijing Medical Journal

摘　　要：目的　探讨急性脑外伤应激性溃疡胃黏膜中巯基物质、丙二醛的改变情况及外源性巯基物质的保护作用。方法　采用改良Allen方法制造大鼠颅脑损伤并发应激性溃疡模型 ,将 73只健康雄性Wistar大鼠随机分为7组 :Ⅰ为正常对照组 ;Ⅱ、Ⅲ、Ⅳ组 (应激组 )为颅脑损伤应激后 3、6、2 4h组 ;Ⅴ、Ⅵ组 (预防 +应激组 )分别为应激前腹腔预防注射生理盐水、硫普罗宁组 ;Ⅶ组为无颅脑损伤应激 ,单纯用硫普罗宁预防组。各预防组在损伤前 1 5min腹腔内分别注射各种治疗药品。测定各组胃黏膜组织中的总蛋白巯基 (T SH )、非蛋白巯基 (NP SH)含量 ;检测各组胃黏膜组织中丙二醛 (MDA)含量 ;计算各组大鼠胃黏膜的溃疡指数 (UI )。结果　颅脑损伤后各应激组大鼠胃黏膜组织中T SH及NP SH含量较对照组明显下降 (P <0 .0 1 ) ,并且在应激后 3h即显著降低 ,此后继续减少但坡度变化较平坦 ,MDA含量及UI较对照组显著增高 ;应激 +外源性巯基预防组胃黏膜组织中T SH和NP SH含量较少受到抑制 ,均明显高于各应激组胃黏膜组织中T SH和NP SH含量 (P <0 .0 1 ) ,并且MDA含量和UI也较各应激组显著降低 (P <0 .0 1 )。结论　大鼠急性颅脑损伤应激后胃黏膜巯基含量明显下降 ,丙二醛含量明显升高 ,可能与氧自由基生成增多有关 ,从而产生黏膜损?Objective To study the cytoprotection effect of sulfhyduyl compounds on stress related mucosal damage in rats with brain trauma.Methods The models of stress related mucosal damage with brain trauma in rats were developed by Allen's method.The healthy male Wistar rats(220～280g Wt) were randomly divided into severn groups:Group Ⅰas normal control; Group Ⅱ,Ⅲ,Ⅳ were 3,6and 24h after the brain injury respectively;GroupⅤ,Ⅵ with normal saline prophylaxis,Tiopronin prophylaxis,before brain injury respectively;GroupⅦ was Tiopronin control without brain injury.The time of administration drug was intraperitoneally 15 minutes before brain injury.Levels of total protein sulfhydryl (T SH ),nonprotein sulfhydryl(NP SH) and malondialdehyde (MDA) in gastric mucosa peior to and 3h,6h,24h post brain trauma were determined;The effect of external sulphydryl compounds on the T SH,NP SH,MDA and UI of gastric mucosa in rats 24h after tstress was estimated.Results ①There was a markedly decrease in T SH and NP SH content in the mucosa of rats (group Ⅱ,Ⅲ,Ⅳ)( P <0.01).The decreased levels commenced significantly within 3h after brain injury( P < 0.01 ),then continuously decreasing,and lasted for 24h.②In group Ⅵ,the decreased levels of T SH,NP SH had been controlled.Levels of T SH and NP SH were increased markedly( P < 0.01 ).While MDA was dcreased significantly ( P <0.01) compared with groupⅡ,Ⅲ,Ⅳ.Conclusions The sharp decrease of the sulfphydryls contents and increase of MDA contents of gastric mucosa are probable involved in the pathogensis of stress related mucosal damage in rats with brain trauma which perhaps induced by much more free radicals that were producted by stress.The external sulfphydryl has cytoprotective effect of gastric mucosa by supplementing internal sulfphydryl which probably can directly or indirectly eliminate oxygen free radicals.

关 键 词：巯基物质 大鼠 颅脑外伤 胃细胞保护作用 颅脑损伤 应激性黏膜损害 

分 类 号：R651.1[医药卫生—外科学]