支气管哮喘患者β_2-肾上腺素能受体基因多态性与血清总IgE关系的研究  

Study on relationship between IgE and β_2-adrenergic receptor genetic polymorphisms in asthmatics

作　　者：张湘燕[1] 冯端兴[1] 叶贤伟[1] 余红[1] 刁晓源[1] 李敏[1] 

机构地区：[1]贵州省人民医院呼吸内科,贵阳550002

出　　处：《贵州医药》2004年第8期693-694,F002,共3页Guizhou Medical Journal

基　　金：贵州省科技厅科研攻关项目 (黔科合 2 0 0 3NGY0 2 1)

摘　　要：目的探讨支气管哮喘患者 β2 肾上腺素能受体 (β2 AR) 16、2 7位点基因多态性与血清总IgE的相关性。方法采用等位基因特异性聚合酶链反应 (AS PCR)方法 ,对 4 4例支气管哮喘患者进行 β2 AR基因多态性分析 ,并测定其血清总IgE。结果各基因型血清总IgE的值 ,16位点 :精氨酸 /精氨酸为 5 2 5 2 5± 2 2 0 73mmol/L ,甘氨酸 /精氨酸为 4 19 87± 2 6 4 0 8mmol/L ,甘氨酸 /甘氨酸为 5 35 10±2 89 5 1mmol/L ,三者比较差异无显著性 (P >0 0 5 ) ;2 7位点 :谷氨酰胺 /谷氨酰胺为 75 6 13±2 2 0 6 3mmol/L ,谷氨酸 /谷氨酰胺为 341 0 0± 136 6 6mmol/L ,谷氨酸 /谷氨酸为 4 2 5 33± 2 74 13mmol/L ;三者比较差异有显著性 (P <0 0 1)。结论 β2 AR基因 2 7位点多态性与血清总IgE相关联。Objective To analyze the association between β 2-AR genetic polymorphism and serum total IgE in asthma patients. Methods AS-PCR were used to determine 16, 27 locus alleles of β 2-AR genetic polymorphisms and ELISA were used to determine serum total IgE in 44 unrelated patients with asthma. Results The relativity between serum total IgE and genotype β 2-AR16 loci: the level of IgE was 525.25±220.73mmol/l in Arg/Arg, 419.87±264.08mmol/l in Arg/Gly and 535.10± 289.51 mmol/l in Gly/Gly respectively. There was no significant difference in serum total IgE compared with three groups. The level of IgE were 756.13±220.63mmol/l in Gln/Glu, 341.00±136.66mmol/l in Gln/Glu and 425.33±274.13mmol/l in Glu/Glu respectively. There were significant difference in β 2-AR 27 locus of serum total IgE compared with three groups. Conclusion β 2-AR 27 locus genetic polymorphism is correlated with serum total IgE in asthma patients.

关 键 词：支气管哮喘 Β2-肾上腺素能受体 基因多态性 血清总IGE Β2-AR 谷氨酸 

分 类 号：R562.25[医药卫生—呼吸系统]