反相高效液相色谱法测定醋酸氢化可的松软膏的含量  

RP - HPLC Determination of Hydrocortisone Acetate Ointment

作　　者：左志辉[1] 周建玉[1] 左文坚[1] 

机构地区：[1]天津市药品检验所,天津300070

出　　处：《药物分析杂志》2004年第4期420-421,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:改进了醋酸氢化可的松软膏的含量测定方法,为其质量控制提供快速、有效的分析方法。方法:色谱柱为DikmaODS柱(4.6 mm×250 mm,5μm),流动相:乙腈-水(38:62),检测波长254 nm,流速:1.0 mL·min-1,柱温:40℃。并进行了方法学验证。结果:醋酸氢化可的松的平均回收率为99.70%(n=9);醋酸氢化可的松浓度在4.5-53.9μg·mL-1范围内呈良好线性关系(r=0.9999)。结论:该方法快速、简单、准确、灵敏、重复性好,并能更好地控制药品质量。Objective: An HPLC method was established to determine hydrocortisone acetate ointment by RP HPLC. Method: A Dikma ODS(4. 6 mm×250 mm,5 μm)column was used with a mobile phase consisted of a mixture of acetonitrile - water(38:62) at a flow rate of 1. 0 m·min-1 and UV detector wavelength at 254 nm. Column temperature was 40 o@. Results: The average recovery of hydrocortisone acetate ( n = 9) was 99. 70% , The linear range of hydrocortisone acetate was 4. 5 -53.9μg·mL-1 ( r - 0. 9999 ). Conclusion: This method is rapid, simple, accurate and sensitive, it can control the quality of hydrocortisone acetate ointment better.

关 键 词：反相高效液相色谱法 醋酸氢化可的松软膏 糖皮质激素 线性试验 含量测定 

分 类 号：R977.11[医药卫生—药品] R914[医药卫生—药学]