不同化学物质对新生大鼠中脑神经前体细胞神经元生成的影响  

Differentiation of mesencephalic progenitors induced by different chemicals in vitro

作　　者：李怡[1] 邢萱[2] 赵仑[3] 娄淑杰[1] 何成[1] 路长林[1] 

机构地区：[1]第二军医大学神经生物学教研室,上海200433 [2]第二军医大学数理教研室 [3]航天医学工程研究所

出　　处：《解剖学杂志》2004年第4期337-340,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金 (39930 0 80 )

摘　　要：目的 :观察新生大鼠中脑神经前体细胞对不同化学物质的反应性 ,以期找到体外有效增加其神经元生成的化学物质。方法 :建立新生大鼠中脑神经前体细胞的培养体系 ,分别被各种化学物质诱导分化 ,利用神经元特异性标记物进行免疫细胞化学染色 ,检测不同化学物质诱导神经元生成的比例。结果 :白介素 1α促新生大鼠中脑神经前体细胞的神经元生成 ,天麻素、胶质源性神经营养因子和睫状神经营养因子呈抑制效应 ,雌二醇、葛根素、牛蒡子苷、人参皂甙Rg1无明显作用。 结论 :新生大鼠中脑神经前体细胞的分化方向可被环境信号调控。白介素 1α是上述化学物质中唯一有望增加植入后神经元产量的细胞因子。Objective: To examine the responsivenss of newborn rat mesencephalic progenitors to different chemicals, looking for a way to efficiently enhance their neurogenesis in vitro. Methods: Newborn rat midbrain-derived neural progenitors were used to examine the effects of different chemicals. Eight different chemicals, glial cell line-derived neurotrophic factor (GDNF),ciliary neurotrophic factor (CNTF),interleukin-1 alpha(IL-1α),puerarin, arctiin ginsenoside Rg1,gastrodin or 17 beta-estradiol,were screened for their ability to induce the mesencephalic progenitors toward neuronal phenotype. Using immunocytochemistry,the percentage of microtubule-associated protein-2ab (a specific neuron marker)-positive cells was calculated. Results: In contrast to spontaneous differentiation,only IL-1α was found to promote neuron-oriented differentiation on mesencephalic progenitors while GDNF,CNTF and gastrodin showed an inhibitory effect, and the rest chemicals had no effect on neuronal differentiation. Conclusion: Fate of newborn rat mesencephalic progenitors can be manipulated by environmental signal in a neuronal phenotype before cell transplantation.Further investigation may contribute to find gene targets to control linage specification of neural progenitors.

关 键 词：化学物质 新生大鼠 脑神经 前体细胞 神经元生成 抑制效应 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]