雌激素对卵巢摘除大鼠心内神经节细胞胆碱乙酰基转移酶和一氧化氮合酶表达的影响  

Effects of estrogen on expression of choline acetyltransferase and nitric oxide synthase 1 in intracardiac ganglionic neurons in ovariectomized rats

作　　者：陈小武[1] 祝善乐[2] 范玉华[2] 赵小明[2] 

机构地区：[1]咸宁学院解剖学教研室,咸宁437100 [2]华中科技大学同济医学院解剖学系

出　　处：《解剖学杂志》2004年第4期408-410,424,共4页Chinese Journal of Anatomy

摘　　要：目的 :探讨雌激素对卵巢摘除大鼠心内神经节细胞中神经递质的影响。方法 :建立去卵巢 (OVX)和雌激素替代疗法动物模型 ,2个月后用免疫组化结合图象分析方法观察大鼠心内神经节细胞中胆碱乙酰基转移酶 (ChAT)和一氧化氮合酶 (NOS1)的变化。结果 :在OVX大鼠心内神经节细胞中ChAT和NOS1较正常对照组明显减少 ,雌激素 (E2 )替代治疗组与正常对照组相比无明显变化 ,OVX +雌激素受体拮抗剂 (TAM)组较正常对照组显著减少 ,但与OVX组无明显差异。结论 :雌激素能上调大鼠 (OVX)心内神经节细胞中ChAT和NOS1的表达 ,雌激素可能够通过对心内神经节细胞中神经递质的调节 。Objective: To investigate the effects of estrogen on the expression of Choline acetyltransferase (ChAT) and nitric oxide synthase 1 (NOS 1) in the intracardiac ganglionic neurons of the OVX rats. Methods: OVX and ERT models were set up and the rats were killed after 2 months.The changes of ChAT and NOS 1 in intracardiac ganglionic neurons was investigated by immunohistochemical method together with image analysis method. Results: The ChAT and NOS 1 levels in the OVX group was evidently lower than those in the normal group, and there was no evident difference between the ERT group and the normal group, but the ChAT and NOS 1 levels in the OVX+TAM+E2 group were lower than those in the normal group. There was no evident difference between the OVX group and the OVX+TAM+E2 group. Conclusion: The results suggest that estrogen can upregulate the expression of ChAT and NOS 1 in the intracardiac ganglionic neurons of the rats,and then indirectly regulates the function of the cardiovascular system.

关 键 词：雌激素 卵巢摘除 大鼠 心内神经节细胞 胆碱乙酰基转移酶 一氧化氮合酶 基因表达 心血管功能 

分 类 号：R965[医药卫生—药理学]