视网膜神经节细胞与Müller细胞共培养的方法研究  

Co-culture of retinal ganglion cells and Müller cells:a method study

作　　者：苟琳[1] 张作明[1] 许汉鹏[2] 郭群[1] 李莉[1] 郭守一[1] 

机构地区：[1]第四军医大学航空航天医学系临床医学教研室,西安710032 [2]第四军医大学神经生物研究所

出　　处：《眼科研究》2003年第3期264-266,共3页Chinese Ophthalmic Research

摘　　要：目的　建立一种简单的体外培养视网膜神经节细胞的方法。方法　采用Thy1 1单克隆抗体免疫吸附得到纯化的视网膜神经节细胞 ,将其接种于纯化培养的视网膜M櫣ｌｌｅｒ细胞上 ,在无血清培养液中共同培养。结果　视网膜神经节细胞在接种后 1d即可生长出短小的突起 ,至第 5d突起不断增长并可长出二级分支。结论　M櫣ｌｌｅｒ细胞在无血清培养液中 ,对体外培养的神经节细胞具有很好的支持营养作用。ObjectiveTo establish a new method for purification culture of retinal ganglion cells in vitro.MethodsRetinal ganglion cells were purified by the immuo absorbtion method with the retinal ganglion cells(RGCs) specific monoclonal antibody Thy1 1.The purified RGCs were seeded on confluent retinal Müller cell that had been obtained by shaken process.ResultsShort neurites outgrowth could be found from the RGCs 1 day after seeding,the neurites continued to grow and grew out the second branches in 5 day.ConclusionThe purified Müller cells can support the growth of RGCs in serum free medium.Müller cells have neurotrophic effect on the RGCs.

关 键 词：视网膜神经节细胞 细胞培养 MÜLLER细胞 Thy1.1 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]