铜(Ⅱ)配合物与DNA作用的光谱法研究  

Studies on the Interaction of Metalic Complex and DNA by the Spectral Methods

作　　者：林秋月[1] 胡瑞定[1] 郑孝华[1] 

机构地区：[1]浙江师范大学化学与生命科学学院化学系,浙江金华321004

出　　处：《光谱学与光谱分析》2004年第8期988-990,共3页Spectroscopy and Spectral Analysis

摘　　要：采用紫外光谱和荧光光谱研究了配离子 [Cu(A) 2 ]2 +(其中A =邻菲络啉 ( phen) ,联吡啶 (bpy) ,乙二胺 (en) )与小牛胸腺DNA的相互作用。实验发现配合物的存在使DNA碱变性的 pH增大 ,变性后增色效应减小。EB DNA体系的荧光强度随 [Cu( phen) 2 ]2 +的加入迅速减弱。在DNA存在下 ,配离子被猝灭剂 [Fe(CN) 6]4-的发光猝灭程度减小。进一步研究了配体分子平面的大小对配合物与DNA作用的影响。结果表明 ,铜配合物与DNA之间发生了插入作用 ,且该作用随配体芳环平面的减小而减弱 ,即 [Cu( phen) 2 ]2 +>[Cu(bpy) 2 ]2 +>[Cu(en) 2 ]2 +。The interaction of [Cu(A)(2)](2+) (A = phen, bpy, en) complex ion and ct-DNA was investigated by using absorption spectra and fluorescence spectra. Based on the measurement of A(260) in the different pH mediums, the alkaline denaturation curves of DNA in the absence and in the presence of [Cu(phen)(2)](2+) complex ion were obtained. The result showed that the alkaline denaturation for [Cu(phen)(2)](2+) complex ion occured at a higher pH than that for DNA alone. The absorption of EB-DNA system in the presence of [Cu(phen)(2)](2+) showed that [Cu(phen)(2)](2+) could partly sustain the place of EB. The result of fluorescence spectra suggested that the [Cu(phen)(2)](2+) complex ion could make the fluorescence intensity of EB-DNA system to decreased sharply. The effect of [Fe(CN)(6)](4-) on the emission of [Cu(phen)(2)](2+) and [Cu(phen)(2)](2+)-DNA system was studied. All the experimental results indicated that the intercalative binding mode was the major mode for the interaction of [Cu(phen)(2)](2+) complex ion and DNA, and the size of ligands can effect the binding of complexes to DNA.

关 键 词：铜配合物 DNA 光谱法 邻菲络啉 联吡啶 乙二胺 插入作用 

分 类 号：Q523[生物学—生物化学] O614.121[理学—无机化学]