黄酮化合物的偶氮显色反应及其应用研究  

Azoic Color Reaction of Flavonoids and Their Analytical Application

作　　者：余倩[1] 陈小康[2] 罗宗铭[1] 杨迟[1] 麦桦坤[1] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510090 [2]韶关学院化学系,广东韶关512005

出　　处：《光谱学与光谱分析》2004年第8期991-994,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金 (2 0 1 0 50 0 3);广东省自然科学基金(980 1 2 8)资助项目

摘　　要：在pH 10 4的NH4Cl NH3 ·H2 O缓冲溶液中 ,对磺基氯化重氮苯可与芦丁 ,桑色素 ,染料木甙 ,槲皮素 ,橙皮甙 ,黄岑甙和大豆甙等 7种黄酮化合物发生偶氮显色反应 ,反应产物的最大吸收波长 ,除黄岑甙为 380nm外 ,其余均在 4 30nm ,显色反应的灵敏度依上述顺序逐渐减弱。其中芦丁的摩尔吸收光系数最大 ,为 3 2 8× 10 4L·mol-1·cm-1,大豆甙摩尔吸光系数最小 ,为 8 30× 10 2 L·mol-1·cm-1。提出的分光光度分析方法 ,用于槐米和豆粕中黄酮化合物的测定 ,RSD为 0 2 5 %～ 4 4 8% ,回收率在 99 3%～ 10 5 0In NH4 Cl-NH3 . H2O buffer solution of, pH 10.4, diazo salt of p-sulfodiazoben-zene chloride reacts with rutin, morin, genistin, quercetin, hesperidin, baicalin and daidzin to form color azo-compounds. These azo-compounds have an absorption maximum at 430 nm except for baicalin azo-compound, which has an absorption maximum at 380 nm. The sensitivity of azoic color reaction is reductive in the order of above flavonoids and the molar absorptivity is maximum for rutin and is minimal for daidzin among these azo-compounds, i.e. 3.28 x 10(4) and 8.3 x 10(2) L.mol(-1) . cm(-1) respectively. The proposed spectrophotometry has been applied to the determination of flavonoids in sophora seed and soybean cake. The RSDs of the method were found to be in the range of 0.75%-4.48%, and the recoveries were in the range of 99.3%-105.0%.

关 键 词：黄酮化合物 偶氮显色反应 分光光度法 槐米 豆粕 

分 类 号：O626[理学—有机化学] O657.3[理学—化学]